Expression of the alpha 5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matrices

Auteur(s): Lake, JenniferZaniolo, KarineGaudreault, ManonCarrier, PatrickDeschambault, AlexandreBazin, RichardGermain, LucieSalesse, ChristianGuérin, Sylvain
Autre(s) titre(s): Expression of the α5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matrices
Résumé: The integrin α5β1 plays a major role in corneal wound healing by promoting epithelial cell adhesion and migration over the fibronectin matrix secreted as a cellular response to corneal damage. Expression of α5 is induced when rabbit corneal epithelial cells (RCECs) are grown in the presence of fibronectin. Here, we examined whether α5 expression is similarly altered when RCECs or human corneal epithelial cells (HCECs) are grown on a reconstructed stromal matrix used as an underlying biomaterial. Mass spectrometry and immunofluorescence analyses revealed that the biomaterial matrix produced by culturing human corneal fibroblasts with ascorbic acid (ECM/35d) contains several types of collagens, fibronectin, tenascin and proteoglycans. Results from transfection of CAT/α5-promoter plasmids, Western blot and EMSA analyses indicated that ECM/35d significantly increase expression of α5 in HCECs as a result of alteration in the expression and DNA binding of the transcription factors NFI, Sp1, AP-1 and PAX6. The biological significance of this biomaterial substitute on the expression of the α5 gene may therefore contribute to better understand the function played by the α5β1 integrin during corneal wound healing.
Type de document: Article de recherche
Date de publication: 30 mai 2013
Date de la mise en libre accès: Accès restreint
Version du document: VoR
Lien permanent: http://hdl.handle.net/20.500.11794/16844
Ce document a été publié dans: Biomaterials, Vol. 34 (27), 6367–6376 (2013)
https://doi.org/10.1016/j.biomaterials.2013.05.007
IPC Science and Technology Press
Autre version disponible: 10.1016/j.biomaterials.2013.05.007
23727260
Collection :Articles publiés dans des revues avec comité de lecture

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