Expression of the alpha 5 integrin gene in corneal epithelial cells cultured on tissue-engineered human extracellular matrices

Authors: Lake, JenniferZaniolo, KarineGaudreault, ManonCarrier, PatrickDeschambault, AlexandreBazin, RichardGermain, LucieSalesse, ChristianGuérin, Sylvain
Abstract: The integrin α5β1 plays a major role in corneal wound healing by promoting epithelial cell adhesion and migration over the fibronectin matrix secreted as a cellular response to corneal damage. Expression of α5 is induced when rabbit corneal epithelial cells (RCECs) are grown in the presence of fibronectin. Here, we examined whether α5 expression is similarly altered when RCECs or human corneal epithelial cells (HCECs) are grown on a reconstructed stromal matrix used as an underlying biomaterial. Mass spectrometry and immunofluorescence analyses revealed that the biomaterial matrix produced by culturing human corneal fibroblasts with ascorbic acid (ECM/35d) contains several types of collagens, fibronectin, tenascin and proteoglycans. Results from transfection of CAT/α5-promoter plasmids, Western blot and EMSA analyses indicated that ECM/35d significantly increase expression of α5 in HCECs as a result of alteration in the expression and DNA binding of the transcription factors NFI, Sp1, AP-1 and PAX6. The biological significance of this biomaterial substitute on the expression of the α5 gene may therefore contribute to better understand the function played by the α5β1 integrin during corneal wound healing.
Document Type: Article de recherche
Issue Date: 30 May 2013
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/16844
This document was published in: Biomaterials, Vol. 34 (27), 6367–6376 (2013)
https://doi.org/10.1016/j.biomaterials.2013.05.007
IPC Science and Technology Press
Alternative version: 10.1016/j.biomaterials.2013.05.007
23727260
Collection:Articles publiés dans des revues avec comité de lecture

Files in this item:
SizeFormat 
2013_Lake(Guerin)_Biomaterials.pdf
1.9 MBAdobe PDF    Request a copy
All documents in CorpusUL are protected by Copyright Act of Canada.