Identification of epithelial stem cells in vivo and in vitro using keratin 19 and BrdU

DC FieldValueLanguage
dc.contributor.authorLarouche, Danielle-
dc.contributor.authorLavoie, Amélie-
dc.contributor.authorPaquet, Claudie-
dc.contributor.authorSimard-Bisson, Carolyne-
dc.contributor.authorGermain, Lucie-
dc.date.accessioned2018-02-01T18:25:40Z-
dc.date.available9999-12-31-
dc.date.issued2010-01-01-
dc.identifier.issn1350-9462-
dc.identifier.urihttp://hdl.handle.net/20.500.11794/16830-
dc.description.abstractProgress in the identification of skin stem cells and the improvement of culture methods open the possibility to use stem cells in regenerative medicine. Based on their quiescent nature, the development of label retention assays allowed the localization of skin stem cells in the bulge region of the pilosebaceous units and in the bottom of rete ridges in glabrous skin. The development of markers such as keratin 19 also permits their study in human tissues. In this chapter, protocols to identify skin stem cells based on their slow-cycling property and their expression of keratin 19 will be described in detail. The methods include the labeling of skin stem cells within mouse or rat tissues in vivo, the labeling of proliferative human cells in vitro using 5-bromo-2-deoxyuridine (BrdU), and the detection of keratin 19 and BrdU by immunofluor- escence or immunoperoxidase staining.fr
dc.format.extentPages 383-400fr
dc.languageengfr
dc.publisherHumana Pressfr
dc.relation.ispartofEpidermal Cellsfr
dc.subjectCell culturefr
dc.subjectEpidermisfr
dc.subjectHair folliclefr
dc.subjectHumanfr
dc.subjectKeratinocytesfr
dc.subjectKeratin 19fr
dc.subjectMousefr
dc.subjectProliferationfr
dc.subjectRatfr
dc.subjectRegenerative medicinefr
dc.subjectStem cellsfr
dc.subjectVibrissafr
dc.titleIdentification of epithelial stem cells in vivo and in vitro using keratin 19 and BrdUfr
dc.typeCOAR1_1::Texte::Livre::Chapitre d'ouvragefr
dc.identifier.doi10.1007/978-1-60761-380-0_27fr
dc.subject.rvmCellules souchesfr
dc.subject.rvmCellules épithélialesfr
dc.subject.rvmKératinefr
dc.subject.rvmBromodésoxyuridinefr
rioxxterms.versionVersion of Recordfr
rioxxterms.version_of_recordhttp://dx.doi.org/10.1007/978-1-60761-380-0_27fr
bul.rights.periodeEmbargoInfinifr
dcterms.publisher.locationNew Yorkfr
Collection:Chapitres de livre

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