Rescue of the transcription factors Sp1 and NFI in human skin keratinocytes through a feeder-layer-dependent suppression of the proteasome activity

Authors: Duval, CélineGaudreault, ManonVigneault, FrançoisTouzel-Deschênes, LydiaRochette, PatrickMasson-Gadais, BénédicteGermain, LucieGuérin, Sylvain
Abstract: Co-culturing human skin keratinocytes along with a feeder layer has proven to considerably improve their proliferative properties by delaying massive induction of terminal differentiation. Through a yet unclear mechanism, we recently reported that irradiated 3T3 (i3T3) fibroblasts used as a feeder layer increase the nuclear content of Sp1, a positive transcription factor (TF) that plays a critical role in many cellular functions including cell proliferation, into both adult skin keratinocytes and newborn skin keratinocytes. In this study, we examined the influence of i3T3 on the expression and DNA binding of NFI, another TF important for cell proliferation and cell cycle progression, and attempted to decipher the mechanism by which the feeder layer contributes at maintaining higher levels of these TFs in skin keratinocytes. Our results indicate that co-culturing both adult skin keratinocytes and newborn skin keratinocytes along with a feeder layer dramatically increases glycosylation of NFI and may prevent it from being degraded by the proteasome.
Document Type: Article de recherche
Issue Date: 12 March 2012
Open Access Date: Restricted access
Document version: VoR
This document was published in: Journal of Molecular Biology, Vol. 418 (5), 281–299 (2012)
Academic Press
Alternative version: 10.1016/j.jmb.2012.01.021
Collection:Articles publiés dans des revues avec comité de lecture

Files in this item:
Description SizeFormat 
1.6 MBAdobe PDF    Request a copy
All documents in CorpusUL are protected by Copyright Act of Canada.