Can we produce a human corneal equivalent by tissue engineering?

Authors: Germain, LucieCarrier, PatrickAuger, François A.Salesse, ChristianGuérin, Sylvain
Abstract: Tissue engineering is progressing rapidly. Bioengineered substitutes are already available for experimental applications and some clinical purposes such as skin replacement. This review focuses on the development of reconstructed human cornea in vitro by tissue engineering. Key elements to consider in the corneal reconstruction, such as the source for epithelial cells and keratocytes, are discussed and the various steps of production are presented. Since one application of this human model is to obtain a better understanding of corneal wound healing, the mechanisms of this phenomenon as well as the function played both by membrane-bound integrins and components from the extracellular matrix have also been addressed. The analysis of integrins by immunohistofluorescence labelling of our reconstructed human cornea revealed that β1, α3, α5, and α6 integrin subunits were expressed but α4 was not. Laminin, type VII collagen and fibronectin were also detected. Finally, the future challenges of corneal reconstruction by tissue engineering are discussed and the tremendous applications of such tissue produced in vitro for experimental as well as clinical purposes are considered.
Document Type: Article de synthèse
Issue Date: 31 July 2000
Open Access Date: Restricted access
Document version: VoR
This document was published in: Progress in Retinal and Eye Research, Vol. 19 (5), 497–527 (2000)
Alternative version: 10.1016/S1350-9462(00)00005-7
Collection:Chapitres de livre

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