Comparison of the pig and feline models for full thickness corneal transplantation

Authors: Brunette, Isabelle; Rosolen, Serge G.; Carrier, Michel; Abderrahman, Mona; Nada, Ossama; Germain, LucieProulx, Stéphanie
Abstract: Purpose: The goal of this study was to report on the advantages and limitations of the pig and feline models for experimental in vivo corneal transplantation. Methods: Ten healthy domestic pigs and ten healthy cats were used. Full thickness penetrating keratoplasty was performed using autologous (eight cases), allogeneic (seven cases) or human xenogeneic (three cases) tissue. In two other cases, the inflammatory response to partial thickness trephination (without transplantation) was evaluated. Eyes were assessed daily before and after surgery by slit-lamp, pachymetry, and tonometry. A transparency score ranging from 0 (opaque graft) to 4 (clear graft) was used, based on the slit-lamp examination. Optical coherence tomography, histology, and electron microscopy were performed postmortem. Results: In the pig, the mean (±SD) transparency score for the eight full thickness grafts was 0.88 ± 0.99, ranging from 0 to 3. In the feline model, the mean transparency score for the seven uncomplicated grafts was 3.93 ± 0.19, ranging from 3.5 to 4. Both negative controls without endothelium remained opaque at all time. Intraoperative tendency for iris incarceration into the wound, rapid corneal swelling, suture cheese wiring, and postoperative intraocular inflammation were the main factors jeopardizing the functional success of the corneal transplant in the pig model. Conclusion: Suboptimal functional results were obtained after full thickness corneal transplantation in the pig model, while in the feline model, the same protocol yielded uneventful surgeries and clear transplants, with functional results similar to those achieved in human subjects.
Document Type: Article de recherche
Issue Date: 14 November 2011
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/16750
This document was published in: Veterinary Ophthalmology, Vol. 14 (6), 365–377 (2011)
https://doi.org/10.1111/j.1463-5224.2011.00886.x
Blackwell Science
Alternative version: 10.1111/j.1463-5224.2011.00886.x
22050735
Collection:Articles publiés dans des revues avec comité de lecture

Files in this item:
SizeFormat 
2011_Brunette(Proulx)_Veterinary Ophthalmology.pdf
1.4 MBAdobe PDF    Request a copy
All documents in CorpusUL are protected by Copyright Act of Canada.