Human keratinocytes respond to direct current stimulation by increasing intracellular calcium : preferential response of poorly differentiated cells

Authors: Dubé, JeanRochette-Drouin, OlivierLévesque, PhilippeGauvin, RobertRoberge, CharlesAuger, François A.; Goulet, Daniel; Bourdages, Michel; Plante, MichelMoulin, VéroniqueGermain, Lucie
Abstract: A direct current (DC) endogenous electric field (EF) is induced in the wound following skin injury. It is potentially implicated in the wound healing process by attracting cells and altering their phenotypes as indicated by the response to an EF of keratinocytes cultured as individual cells. To better define the signalization induced by a direct current electric field (DCEF) in human keratinocytes, we took advantage of an in vitro model more representative of the in vivo situation since it promotes cell–cell interactions and stratification. Human keratinocytes were grown into colonies. Their exposure to a DCEF of physiological intensity induced an increase of intracellular calcium. This variation of intracellular calcium resulted from an extracellular calcium influx and was mediated, at least in part, by the L-type voltage-gated calcium channel. The increase in intracellular calcium in response to a DCEF was however not observed in all the cells composing the colonies. The intracellular calcium increase was only detected in keratinocytes that didn't express involucrin, a marker of differentiated cells. These results indicate that DCEF is able to induce a specific calcium response in poorly differentiated keratinocytes. This study brings a new perspective for the understanding of the signaling mechanism of endogenous EF in reepithelialization, a critical process during skin wound healing.
Document Type: Article de recherche
Issue Date: 24 February 2012
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/16737
This document was published in: Journal of Cellular Physiology, Vol. 227 (6), 2660–2667 (2012)
https://doi.org/10.1002/jcp.23008
Liss
Alternative version: 10.1002/jcp.23008
21882192
Collection:Articles publiés dans des revues avec comité de lecture

Files in this item:
SizeFormat 
2012_Dubé_JCellPhys Calcium.pdf
353.29 kBAdobe PDF    Request a copy
All documents in CorpusUL are protected by Copyright Act of Canada.