Tissue Engineering of Feline Corneal Endothelium Using a Devitalized Human Cornea as Carrier

DC FieldValueLanguage
dc.contributor.authorProulx, Stéphanie-
dc.contributor.authorAudet, Caroline-
dc.contributor.authorUwamaliya, Jeanne d'Arc-
dc.contributor.authorDeschambeault, Alexandre-
dc.contributor.authorCarrier, Patrick-
dc.contributor.authorGiasson, Claude-J.-
dc.contributor.authorBrunette, Isabelle-
dc.contributor.authorGermain, Lucie-
dc.description.abstractThe difficulties in obtaining good quality tissue for the replacement of corneas of patients suffering from endothelial dysfunctions have prompted us to evaluate the feasibility of producing a tissue-engineered (TE) corneal endothelium using devitalized human stromal carriers. Thus, corneal substitutes were produced by seeding cultured feline corneal endothelial cells on top of previously frozen human corneal stromas. After two weeks of culture to allow attachment and spreading of the seeded cells, the TE corneal endothelium was stained with alizarin red for endothelial cell count and fixed for histology, immunofluorescence labeling, scanning and transmission electron microscopy. Histology and Hoechst staining showed that there were no remaining cells in the devitalized stroma. After seeding, histology and transmission electron microscopy showed that the TE corneal endothelium formed a monolayer of tightly packed cells that were well adhered to Descemet’s membrane. Scanning electron microscopy corroborated that the cells covered the entire posterior corneal surface and had an endothelial morphology. Alizarin staining showed that mean cell counts were 2272 ± 344 cells=mm2 , indicating that the cell density was appropriate for grafting. The TE feline corneal endothelium also expressed the function-related proteins Na+ =HCO3 , ZO-1, and Na+ =K+ -ATPase a1, and could easily be marked with a fluorescent tracker. This study demonstrates the feasibility of reconstructing a highly cellular and healthy corneal endothelium on devitalized human corneal stromas.fr
dc.publisherMary Ann Liebert, Inc. Publishersfr
dc.titleTissue Engineering of Feline Corneal Endothelium Using a Devitalized Human Cornea as Carrierfr
dc.typeCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationTissue Engineering, Part A, Vol. 15 (7), 1709-1718 (2009)fr
dc.subject.rvmGénie tissulaire -- Modèles animauxfr
dc.subject.rvmÉpithélium antérieur de la cornéefr
dc.subject.rvmChats (Animaux de laboratoire)fr
rioxxterms.versionVersion of Recordfr
rioxxterms.project.funder_nameCanadian Institutes of Health Researchfr
rioxxterms.project.funder_nameFonds de Recherche du Québec - Santéfr
rioxxterms.project.funder_nameResearch in Vision Health Network, Montréal, Canadafr
rioxxterms.project.funder_nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.project.funder_nameCharles-Albert Poissant Research Chair in Corneal Transplantation, University of Montrealfr
Collection:Articles publiés dans des revues avec comité de lecture

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