Applications of human tissue-engineered blood vessel models to study the effects of shed membrane microparticles from T-Lymphocytes on vascular function

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dc.contributor.authorPricci, Maria-
dc.contributor.authorBourget, Jean-Michel-
dc.contributor.authorRobitaille, Hubert-
dc.contributor.authorPorro, Chiara-
dc.contributor.authorSoleti, Raffaella-
dc.contributor.authorMostefai, Ahmed-
dc.contributor.authorAuger, François A.-
dc.contributor.authorMartinez, Carmen-
dc.contributor.authorAndriantsitohaina, Ramaroson-
dc.contributor.authorGermain, Lucie-
dc.description.abstractMicroparticles (MPs) are membrane vesicles harboring cell surface proteins and containing cytoplasmic components of the original cell. High levels of circulating MPs have been detected in pathological states associated with vascular dysfunction. We took advantage of the self-assembly method of tissue engineering to produce in vitro three vascular constructs from human vascular smooth muscle cells and fibroblasts to investigate the role of the adventitia in the modulation of vascular tone by MPs, comparing the contractile response of each of these constructs to histamine. The first two were composed of an adventitia (tissue-engineered vascular adventitia (TEVA)) or a media (tissue-engineered vascular media (TEVM)) solely, and the third one contained a media and an adventitia (tissue-engineered vascular media and adventitia (TEVMA)). In the three constructs, the results show that histamine induces contraction insensitive to blockade of inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) and not affected by MP treatment. MPs decreased NO production and nuclear factor (NF)-kB expression but did not affect superoxide anion (O2) release in TEVA. MPs enhanced NF-kB expression but did not affect iNOS and COX-2 expression or NO or O2 release in TEVM. In TEVMA, MPs did not enhance NF-kB expression, but COX-2 expression was higher, and O2 release was lower. Thus, MPs affected NO, O2, NF-kB, and COX-2 in a subtle fashion to maintain the contractile response to histamine. The use of tissue-engineered vascular constructs results in a better understanding of the effect of MPs on human adventitia and
dc.publisherMary Ann Liebert, Inc. Publishersfr
dc.subjectAdventice (Histologie)fr
dc.titleApplications of human tissue-engineered blood vessel models to study the effects of shed membrane microparticles from T-Lymphocytes on vascular functionfr
dc.typeCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationTissue Engineering, Part A, Vol. 15 (1), 137-145 (2009)fr
dc.subject.rvmVaisseaux sanguinsfr
dc.subject.rvmMicroparticules membranairesfr
dc.subject.rvmLymphocytes Tfr
dc.subject.rvmGénie tissulairefr
rioxxterms.versionVersion of Recordfr
rioxxterms.project.funder_nameCanadian Institutes of Health Researchfr
rioxxterms.project.funder_nameFonds de Recherche du Québec - Santéfr
rioxxterms.project.funder_nameInstitut National de la Santé et de la Recherche Médicalefr
rioxxterms.project.funder_nameCanadian Research Chair on Stem Cells and Tissue Engineeringfr
Collection:Articles publiés dans des revues avec comité de lecture

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