Applications of human tissue-engineered blood vessel models to study the effects of shed membrane microparticles from T-Lymphocytes on vascular function

Authors: Pricci, Maria; Bourget, Jean-MichelRobitaille, Hubert; Porro, Chiara; Soleti, Raffaella; Mostefai, Ahmed; Auger, François A.; Martinez, Carmen; Andriantsitohaina, Ramaroson; Germain, Lucie
Abstract: Microparticles (MPs) are membrane vesicles harboring cell surface proteins and containing cytoplasmic components of the original cell. High levels of circulating MPs have been detected in pathological states associated with vascular dysfunction. We took advantage of the self-assembly method of tissue engineering to produce in vitro three vascular constructs from human vascular smooth muscle cells and fibroblasts to investigate the role of the adventitia in the modulation of vascular tone by MPs, comparing the contractile response of each of these constructs to histamine. The first two were composed of an adventitia (tissue-engineered vascular adventitia (TEVA)) or a media (tissue-engineered vascular media (TEVM)) solely, and the third one contained a media and an adventitia (tissue-engineered vascular media and adventitia (TEVMA)). In the three constructs, the results show that histamine induces contraction insensitive to blockade of inducible nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) and not affected by MP treatment. MPs decreased NO production and nuclear factor (NF)-kB expression but did not affect superoxide anion (O2) release in TEVA. MPs enhanced NF-kB expression but did not affect iNOS and COX-2 expression or NO or O2 release in TEVM. In TEVMA, MPs did not enhance NF-kB expression, but COX-2 expression was higher, and O2 release was lower. Thus, MPs affected NO, O2, NF-kB, and COX-2 in a subtle fashion to maintain the contractile response to histamine. The use of tissue-engineered vascular constructs results in a better understanding of the effect of MPs on human adventitia and media.
Document Type: Article de recherche
Issue Date: 15 October 2008
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/16729
This document was published in: Tissue Engineering, Part A, Vol. 15 (1), 137-145 (2009)
https://doi.org/10.1089/ten.tea.2007.0360
Mary Ann Liebert, Inc. Publishers
Alternative version: 10.1089/ten.tea.2007.0360
18925833
Collection:Articles publiés dans des revues avec comité de lecture

Files in this item:
SizeFormat 
2009_Pricci_TEngpartA.pdf
4.79 MBAdobe PDF    Request a copy
All documents in CorpusUL are protected by Copyright Act of Canada.