Mechanical properties of endothelialized fibroblast-derived vascular scaffolds stimulated in a bioreactor

Authors: Tondreau, MaximeLaterreur, VéroniqueGauvin, RobertVallières, KarineBourget, Jean-MichelLacroix, DanTremblay, CatherineGermain, LucieRuel, JeanAuger, François A.
Abstract: There is an ongoing clinical need for tissue-engineered small-diameter (<6 mm) vascular grafts since clinical applications are restricted by the limited availability of autologous living grafts or the lack of suitability of synthetic grafts. The present study uses our self-assembly approach to produce a fibroblast-derived decellularized vascular scaffold that can then be available off-the-shelf. Briefly, scaffolds were produced using human dermal fibroblasts sheets rolled around a mandrel, maintained in culture to allow for the formation of cohesive and three-dimensional tubular constructs, and then decellularized by immersion in deionized water. Constructs were then endothelialized and perfused for 1 week in an appropriate bioreactor. Mechanical testing results showed that the decellularization process did not influence the resistance of the tissue and an increase in ultimate tensile strength was observed following the perfusion of the construct in the bioreactor. These fibroblast-derived vascular scaffolds could be stored and later used to deliver readily implantable grafts within 4 weeks including an autologous endothelial cell isolation and seeding process. This technology could greatly accelerate the clinical availability of tissue-engineered blood vessels.
Document Type: Article de recherche
Issue Date: 6 March 2015
Open Access Date: Restricted access
Document version: VoR
This document was published in: Acta Biomaterialia, Vol. 18, 176–185 (2015)
Elsevier BV
Alternative version: 10.1016/j.actbio.2015.02.026
Collection:Articles publiés dans des revues avec comité de lecture

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