The mitogen-activated protein kinase kinase kinase dual leucine zipper-bearing kinase (DLK) acts as a key regulator of keratinocyte terminal differentiation.

DC FieldValueLanguage
dc.contributor.authorRobitaille, Hubert-
dc.contributor.authorProulx, Roxanne-
dc.contributor.authorRobitaille, Karine-
dc.contributor.authorBlouin, Richard-
dc.contributor.authorGermain, Lucie-
dc.contributor.authorGermain, Lucie-
dc.date.accessioned2018-01-26T16:14:20Z-
dc.date.available2018-01-26T16:14:20Z-
dc.date.issued2005-01-28-
dc.identifier.issn00219258fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/16685-
dc.description.abstractIn the skin, epithelial cells undergo a terminal differentiation program leading to the formation of the stratum corneum. Although it is expected that the last phases of this process must be tightly regulated since it results in cell death, the signaling pathways involved in this induction remain ill defined. We now report that a single kinase, the mitogen-activated protein kinase kinase kinase dual leucine zipper-bearing kinase (DLK), acts in the epidermis to promote the terminal differentiation of human keratinocytes. In support of this notion, we showed that DLK expression was restricted to the granular layer in situ. In addition, cultured keratinocytes infected with a recombinant adenovirus expressing DLK exhibited morphological and biochemical changes, including a suprabasal localization, altered cell shape, compacted cytoplasm, DNA fragmentation, and the up-regulation of filaggrin, that are reminiscent of a terminally differentiated phenotype. Moreover the expression of wild-type DLK in keratinocytes stimulated transglutaminase activity and the consequent formation of the cornified cell envelope, while a kinase-inactive variant of DLK did not. Together these results identify DLK as a signaling molecule implicated in the regulation of keratinocyte terminal differentiation and cornification.fr
dc.languageengfr
dc.publisherAmerican Society for Biochemistry and Molecular Biologyfr
dc.titleThe mitogen-activated protein kinase kinase kinase dual leucine zipper-bearing kinase (DLK) acts as a key regulator of keratinocyte terminal differentiation.fr
dc.typeCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationThe Journal of biological chemistry, Vol. 280 (13), 12732-12741 (2005)fr
dc.identifier.doi10.1074/jbc.M411619200fr
dc.identifier.pubmed15695824fr
dc.subject.rvmKératinocytesfr
dc.subject.rvmMAP kinase kinase kinasesfr
dc.subject.rvmLeucinefr
rioxxterms.versionVersion of Recordfr
rioxxterms.version_of_recordhttps://doi.org/10.1074/jbc.M411619200fr
bul.rights.periodeEmbargo12 moisfr
dc.audience.peerreviewOuifr
Collection:Articles publiés dans des revues avec comité de lecture

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