A tissue-engineered endothelialized dermis to study the modulation of angiogenic and angiostatic molecules on capillary-like tube formation in vitro

Authors: Hudon, ValérieBerthod, FrançoisBlack, Annie; Damour, Odile; Germain, LucieAuger, François A.
Abstract: Background: Because angiogenesis is a major feature of different physiological and pathological situations, the identification of factors that stimulate or inhibit this process and the elucidation of their mechanisms of action are most certainly of clinical relevance. We have produced a new model of endothelialized reconstructed dermis that promotes the spontaneous formation of a human capillary-like network and its stabilization in vitro for a period longer than 1 month. Objectives: The aim of this work was to describe the three-dimensional structure of the capillarylike network. Thereafter we strove to study, quantitatively and qualitatively, the influence of angiogenic and angiostatic drugs on capillary-like tube (CLT) formation in vitro in the model. Methods: The endothelialized dermis was prepared by coculturing two human cell types, dermal fibroblasts and umbilical vein endothelial cells, in a collagen sponge biomaterial. Results: The visualization by confocal microscopy of the tubes present in the model showed that the endothelial structures were not cord-like but rather CLTs with well-defined lumina. Moreover, these tubes were organized in a complex network of branching structures. When angiogenic factors (vascular endothelial growth factor 10 ng mL-1 or basic fibroblast growth factor 10 ng mL-1) were added to the model, 1Æ8 and 1Æ4 times more capillaries, respectively, were observed, whereas the addition of progesterone (10 lg mL-1) reduced by 2Æ4 times the number of tubes compared with the control. Conclusions: These results suggest that this model is a highly efficient assay for the screening of potentially angiogenic and angiostatic compounds.
Document Type: Article de recherche
Issue Date: 27 June 2003
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/16684
This document was published in: British Journal of Dermatology, Vol. 148 (6), 1094–1104 (2003)
Blackwell Scientific
Alternative version: 10.1046/j.1365-2133.2003.05298.x
Collection:Articles publiés dans des revues avec comité de lecture

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