Simultaneous isolation of keratinocytes and fibroblasts from a human cutaneous biopsy for the production of autologous reconstructed skin

Authors: Rochon, Marie-HélèneGauthier, Marie JoséeAuger, François A.Germain, Lucie
Abstract: The key step In the reconstruction of skin by the self-assembly approach is to use fibroblasts capable of secreting a mature extracellular matrix and keratinocytes that can associate with one another to form a stratified, differentiated epidermis. To determine the most efficient way to extract both cell types from a single cutaneous biopsy, five different enzymatic combinations were tested. The yield, viability and morphology of the recuperated cells were compared. The length and temperature of the enzymatic treatments influenced the number and the type of cells recuperated. The cells were then cultured in appropriate media in monolayers and reconstructed skin were produced using the self-assembly approach. No difference was observed between the different strains of cells.
Document Type: Article de recherche
Issue Date: 1 August 2001
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/16663
This document was published in: The Canadian journal of chemical engineering, Vol. 79 (4), 663–667 (2001)
https://doi.org/10.1002/cjce.5450790427
John Wiley & Sons
Alternative version: 10.1002/cjce.5450790427
Collection:Articles publiés dans des revues avec comité de lecture

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