Mechanical loading modulates the differentiation state of vascular smooth muscle cells

Authors: Grenier, GuillaumeRémy-Zolghadri, MurielleBergeron, FrançoisGuignard, RinaBaker, KathleenLabbé, RaymondAuger, François A.Germain, Lucie
Abstract: The cause underlying the onset of stenosis after vascular reconstruction is not well understood. In the present study, we evaluated the effect of mechanical unloading on the differentiation state of human vascular smooth muscle cells (hVSMCs) using a tissue-engineered vascular media (TEVM). hVSMCs cultured in a mechanically loaded three-dimensional environment, known as a living tissue sheet, had a higher differentiated state than cells grown on plastic. When the living tissue sheet was detached from its support, the release of the residual stress resulted in a mechanical unloading and cells within the extracellular matrix (ECM) dedifferentiated as shown by downregulation of differentiation markers. The relaxed living tissue sheet can be rolled onto a tubular mandrel to form a TEVM. The rolling procedure resulted in the reintroduction of a mechanical load leading to a cohesive compacted tissue. During this period, cells gradually redifferentiated and aligned circumferentially to the tubular support. Our results suggest that differentiation of hVSMCs can be driven by mechanical loading and may occur simultaneously in the absence of other cell types. The extrapolation of our results to the clinical context suggests the hypothesis that hVSMCs may adopt a proliferative phenotype resulting from the mechanical unloading of explanted blood vessels during vascular reconstruction. Therefore, we propose that this mechanical unloading may play an important role in the onset of vascular graft stenosis.
Document Type: Article de recherche
Issue Date: 24 November 2006
Open Access Date: Restricted access
Document version: VoR
This document was published in: Tissue Engineering, Vol. 12 (11), 3159-3170 (2006)
Mary Ann Liebert
Alternative version: 10.1089/ten.2006.12.3159
Collection:Articles publiés dans des revues avec comité de lecture

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