A novel approach for studying angiogenesis : a human skin equivalent with a capillary-like network

Auteur(s): Black, AnnieHudon, ValérieGermain, LucieAuger, François A.
Résumé: Angiogenesis results from an ordered set of events that can be modulated in vivo by a variety of angiogenesis-enhancing or inhibiting agents. We review in vitro angiogenesis models and the agents that enhance or inhibit angiogenesis. We also discuss a new in vitro angiogenesis model created within a skin equivalent. Briefly, endothelial cells were combined with the cutaneous cells of a standard skin equivalent and cultured in a chitosan cross-linked collagen-glycosaminoglycan scaffold of this endothelialized skin. This model enables the formation of capillary-like structures in a coculture environment containing newly synthesized extracellular matrix by fibroblasts and keratinocytes. Several morphological characteristics associated with the microvasculature in vivo were observed in the endothelialized skin equivalent such as histotypic organization of tubular structures, basement membrane deposition, and intercellular junction formation.
Type de document: Article de recherche
Date de publication: 1 avril 1999
Date de la mise en libre accès: Accès restreint
Version du document: VoR
Lien permanent: http://hdl.handle.net/20.500.11794/16610
Ce document a été publié dans: Cell Biology and Toxicology, Vol. 15 (2), 81-90 (1999)
https://doi.org/10.1023/A:1007541713398
Princeton Scientific Publishers
Autre version disponible: 10.1023/A:1007541713398
10408355
Collection :Articles publiés dans des revues avec comité de lecture

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