Mechanisms of wound reepithelialization : hints from a tissue-engineered reconstructed skin to long-standing questions

Authors: Laplante, AlainGermain, LucieAuger, François A.Moulin, Véronique
Abstract: Wound closure of epithelial tissues must occur efficiently to restore rapidly their barrier function. We have developed a tissue-engineered woundhealing model composed of human skin keratinocytes and fibroblasts to better understand the mechanisms of reepithelialization. It allowed us to quantify the reepithelialization rate, which was significantly accelerated in the presence of fibrin or platelet-rich plasma. The reepithelialization of these 6 mm excisional wounds required the contribution of keratinocyte proliferation, migration, stratification, and differentiation. The epidermis regenerated progressively from the surrounding wound margins. After 3 days, the neoepidermis showed a complete spectrum of changes. Near the wound margin, the differentiation of the neoepidermis (keratins 1/10, filaggrin, and loricrin) and regeneration of the dermoepidermal junction (laminin 5 and collagen IV) were more advanced than toward the wound center, where the proliferative index was significantly increased. The spatial distribution of keratinocytes distinguished by particular features suggests two complementary mechanisms of reepithelialization: 1) the passive displacement of the superficial layers near the wound margin that would rapidly regenerate a barrier function and 2) the crawling of keratinocytes over each other at the tip of the progressing neoepidermis. Therefore, this study brings a new perspective to long-standing questions concerning wound reepithelialization.— Laplante, A. F., Germain, L., Auger, F. A., Moulin, V. Mechanisms of wound reepithelialization: hints from a tissue-engineered reconstructed skin to long-standing questions.
Document Type: Article de recherche
Issue Date: 1 November 2001
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/16581
This document was published in: The FASEB journal, Vol. 15 (13), 2377-2389 (2001)
https://doi.org/10.1096/fj.01-0250com
Federation of American Societies for Experimental Biology
Alternative version: 10.1096/fj.01-0250com
11689463
Collection:Articles publiés dans des revues avec comité de lecture

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