DifferentiaI expression of collagens XII and XIV in human skin and in reconstructed skin

Authors: Berthod, FrançoisGermain, LucieGuignard, Rina; Lethias, Claire; Garrone, Robert; Damour, Odile; Rest, Michel van der; Auger, François A.
Abstract: Collagens XII and XIV localize near the surface of collagen fibrils and may be involved in epithelial-mesenchymal interactions as well as in the modulation of tissue biomechanical properties. Moreover, human skin fibroblasts cultured in monolayer are known to lose their ability to produce collagen XIV and to switch the transcription of collagen XII from the small splice variant (220 kDa) to the large (320 kDa), whereas the small form is the main form found in human skin. We have investigated the expression patterns of these two molecules in human skin as a function of donor age and anatomic site, by using immunohistology with specific monoclonal antibodies. We demonstrated changes in the expression patterns of collagens XII and XIV in human skin after birth. Moreover, in adult scalp skin, very strong staining of collagen XII fibril bundles was observed around hair follicles, in association with very low expression of collagen XW. We also investigated the expression of collagens XII and XIV by fibroblasts and keratinocytes cultured in a reconstructed skin. In these culture conditions, fibroblasts recovered theft ability to produce collagen XIV and re-expressed the small splice variant of collagen XII. These results could be explained by the deposition of large amounts of collagen fibrils by fibroblasts in this culture system. Thus, the re-expression of these collagens suggests that the deposition of banded collagen fibrils is a pre-requisite for the expression of collagen XIV and small variant of collagen XII.
Document Type: Article de recherche
Issue Date: 1 May 1997
Open Access Date: Restricted access
Document version: VoR
Permalink: http://hdl.handle.net/20.500.11794/16569
This document was published in: Journal of investigative dermatology, Vol 108 (5), 737-742 (1997)
https://doi.org/10.1111/1523-1747.ep12292122
Elsevier
Alternative version: 10.1111/1523-1747.ep12292122
9129225
Collection:Articles publiés dans des revues avec comité de lecture

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