Use of human vessels and human vascular smooth muscle cells in pharmacology

Authors: Stoclet, Jean-Claude; Andriantsitohaina, Ramaroson; L'Heureux, Nicolas; Martinez, C.; Germain, LucieAuger, François A.
Abstract: Relatively limited information is available regarding the mechanisms controlling vasomotricity in human vessels. Isolated vessels obtained from patients undergoing surgery were used to characterize the role of endothelial factors and to study coupling mechanisms between receptors, intracellular calcium, and contraction. However, these investigations are limited by the availability of tissues and many uncontrolled factors. Cultured human vascular cells were also used, were these cells rapidly lose at least some of their differentiated characters. Recently, a human blood vessel equivalent was constructed in vitro from cultured cells, using tissue engineering. This technique allowed us to obtain vessel equivalents containing intima, media, and adventitia layers or tubular media layer only. Contraction and rises in intracellular calcium produced by agonists were studied, indicating that such human vessel equivalents may provide valuable models for pharmacological studies.
Document Type: Article de recherche
Open Access Date: Restricted access
Document version: VoR
This document was published in: Cell Biology and Toxicology, Vol. 12 (4), 223–225 (1996)
Princeton Scientific Publishers
Alternative version: 10.1007/BF00438149
Collection:Articles publiés dans des revues avec comité de lecture

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