Induction of DnaK upon γ-irradiation at high and low rates in Escherichia coli

Authors: Saucier, Linda; Dubé, Claudine; Guernec, Anthony; Naim, Fadia
Other Title(s): Induction of DnaK upon gamma-irradiation at high and low rates in Escherichia coli
Abstract: DnaK is implicated in protein folding, repair and degradation. Its protective role during heat shock is well documented and many other stresses can also induce its production. Using a competitive ELISA, intracellular DnaK concentrations were determined in Escherichia coli ATCC 25922 exposed to a γ-irradiation dose of 0.3 KGy applied either at high (8 × 10−2 KGy/min) or low rates (3 × 10−3 KGy/min) and with or without a recuperation period of 22 h at 37˚C post-treatment. All four irradiation treatments reduced cell counts similarly and significantly compared to the control (P < 0.0001). However, the highest DnaK concentration was observed in cells irradiated at low rate without recuperation (105,416 molecules/cell; P = 0.0001). Furthermore, DnaK levels remained higher than the control (38,500 molecules/ cell) after the recuperation period (P < 0.05). Variation in the intracellular DnaK concentration indicates that the bacterial stress response was modulated differently according to the irradiation treatment (P = 0.0001).
Document Type: Article de recherche
Issue Date: 1 October 2012
Open Access Date: 22 January 2018
Document version: VoR
This document was published in: Food and Nutrition Sciences, Vol. 3 (10), 1349-1353 (2012)
Scientific Research Publishing
Alternative version: 10.4236/fns.2012.310178
Collection:Articles publiés dans des revues avec comité de lecture

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