Induction of DnaK upon γ-irradiation at high and low rates in Escherichia coli
Authors: | Saucier, Linda; Dubé, Claudine; Guernec, Anthony; Naim, Fadia |
Other Title(s): | Induction of DnaK upon gamma-irradiation at high and low rates in Escherichia coli |
Abstract: | DnaK is implicated in protein folding, repair and degradation. Its protective role during heat shock is well documented and many other stresses can also induce its production. Using a competitive ELISA, intracellular DnaK concentrations were determined in Escherichia coli ATCC 25922 exposed to a γ-irradiation dose of 0.3 KGy applied either at high (8 × 10−2 KGy/min) or low rates (3 × 10−3 KGy/min) and with or without a recuperation period of 22 h at 37˚C post-treatment. All four irradiation treatments reduced cell counts similarly and significantly compared to the control (P < 0.0001). However, the highest DnaK concentration was observed in cells irradiated at low rate without recuperation (105,416 molecules/cell; P = 0.0001). Furthermore, DnaK levels remained higher than the control (38,500 molecules/ cell) after the recuperation period (P < 0.05). Variation in the intracellular DnaK concentration indicates that the bacterial stress response was modulated differently according to the irradiation treatment (P = 0.0001). |
Document Type: | Article de recherche |
Issue Date: | 1 October 2012 |
Open Access Date: | 22 January 2018 |
Document version: | VoR |
Permalink: | http://hdl.handle.net/20.500.11794/16545 |
This document was published in: | Food and Nutrition Sciences, Vol. 3 (10), 1349-1353 (2012) https://doi.org/10.4236/fns.2012.310178 Scientific Research Publishing |
Alternative version: | 10.4236/fns.2012.310178 |
Collection: | Articles publiés dans des revues avec comité de lecture |
Files in this item:
Description | Size | Format | ||
---|---|---|---|---|
DnaK_FNS 2012 Corpus Open Access.pdf | 607.87 kB | Adobe PDF | ![]() View/Open |
All documents in CorpusUL are protected by Copyright Act of Canada.