Morphologic and functional properties of bronchial cells isolated from normal and asthmatic subjects

Authors: Goulet, FrancineBoulet, Louis-PhilippeChakir, JamilaTremblay, NathalieDubé, JeanLaviolette, MichelBoutet, MichelXu, WenGermain, LucieAuger, François A.
Abstract: Recent advances in biomedical sciences have led to the development of various methods for the evaluation of the physiopathology of respiratory diseases. This study reports morphologic and functional features of cells isolated by a new method from bronchial biopsies of normal and asthmatic subjects. Both epithelial and fibroblastic cells were isolated from the same biopsies using collagenase. The cells were cultured for several passages and stored frozen. Two selective culture media were used in order to obtain pure epithelial and fibroblastic cell populations. Immunofluorescence analysis of intermediate filaments, keratins, and vimentin confirmed the type of the isolated cells. The proportions of alpha-actin-expressing cells varied among the fibroblastic cell populations isolated from normal and asthmatic subjects. Interestingly, the population containing high numbers of alpha-actin-expressing cells and presenting the fastest collagen contraction kinetic was isolated from bronchial biopsies of an asthmatic subject. Moreover, the fibroblastic cells that showed the best contractile properties 24 h after their seeding in floating collagen gels were isolated from bronchial biopsies of asthmatic patients having PC20 values below 1 mg/ml. On the basis of these data, we propose a new approach to isolate, culture and characterize human bronchial cells in vitro.
Document Type: Article de recherche
Issue Date: 1 September 1996
Open Access Date: 18 January 2018
Document version: VoR
This document was published in: American Journal of Respiratory Cell and Molecular Biology, Vol. 15 (3), 312-318 (1996)
American Thoracic Society
Alternative version: 10.1165/ajrcmb.15.3.8810634
Collection:Articles publiés dans des revues avec comité de lecture

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