Skin equivalent produced with human collagen

Authors: Auger, François A.López Valle, Carlos AntonioGuignard, RinaTremblay, NathalieNoël, BernardGoulet, FrancineGermain, Lucie
Abstract: Several studies have recently been conducted on cultured skin equivalent (SE), prepared using human keratinocytes seeded on various types of dermal equivalents (DE). We previously showed the advantages of our anchorage method in preventing the severe surface reduction of DE due to fibroblast contractile properties in vitro. A new anchored human SE was established in our laboratory in order to obtain a bioengineered tissue that would possess the appropriate histological and biological properties. In order to compare the effects of different collagen origins on the evolution of SE in vitro, human keratinocytes were seeded on three types of anchored DE. A comparative study was carried out between bovine SE (bSE), human SE (hSE), and human skin equivalent containing additional dermal matrix components (hSE +). Immunohistological analysis showed that hSE and hSE+ presented good structural organization, including the deposition of several basement membrane constituents. Higher amounts of transglutaminase, ceramides, and keratin 1 were detected in the epidermal layers of all SE when cultured at the air-liquid interface. However, a 92 kDa gelatinase activity was higher in bovine skin equivalent (bSE) compared to hSE cultures. The use of human collagens comparatively to bovine collagen as SE matricial component delayed the degradation of the dermal layer in culture.
Document Type: Article de recherche
Issue Date: 1 June 1995
Open Access Date: Restricted access
Document version: VoR
This document was published in: In Vitro Cellular and Developmental Biology - Animal, Vol. 31 (6), 432–439 (1995)
Society for In Vitro Biology
Alternative version: 10.1007/BF02634255
Collection:Articles publiés dans des revues avec comité de lecture

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