Grafting on nude mice of living skinquivalents produced using human collagens

Authors: López Valle, Carlos AntonioGermain, LucieRouabhia, MahmoudXu, WenGuignard, RinaGoulet, FrancineAuger, François A.
Abstract: Autologous epidermal transplantation for human burn management is an example of a significant breakthrough in tissue engineering. However, the main drawback with this treatment remains the fragility of these grafts during and after surgery. A new human bilayered skin equivalent (hSE) was produced in our laboratory to overcome this problem. The aim of the present work was to study skin regeneration after hSE grafting onto nude mice. A comparative study was carried out over a period of 90 days, between anchored bovine skin equivalent, hSE and hSE+, the latter containing additional matrix components included at concentrations similar to those in human skin in vivo. The addition of a dermal layer to the epidermal sheet led to successful graft take, enhanced healing, and provided mechanical resistance to the grafts after transplantation. In situ analysis of the grafts showed good ultrastructural organization, including the deposition of a continuous basement membrane 1 week after surgery.
Document Type: Article de recherche
Issue Date: 15 August 1996
Open Access Date: 16 January 2018
Document version: AM
Permalink: http://hdl.handle.net/20.500.11794/16461
This document was published in: Transplantation, vol. 62 (3), 317-323 (1996)
http://journals.lww.com/transplantjournal/toc/1996/08150
Williams & Wilkins Co
Alternative version: 8779676
Collection:Articles publiés dans des revues avec comité de lecture

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