Functional evaluation of anchored skin equivalent cultured in vitro : percutaneous absorption studies and lipid analysis

Authors: Michel, MartineGermain, LucieBélanger, Pierre MaximeAuger, François A.
Abstract: In vitro cutaneous models, resulting from recent progress in human cell culture, provide new tools to investigate the development, physiology and pharmacology of the human skin. Indeed, several skin equivalents (SE) have been produced by seeding keratinocytes upon various dermal substrates. One consists of de-epidermized dermis without living fibroblasts but exhibiting an intact basement membrane (1,2). Living dermal models could be obtained in vitro by growing dermal fibroblasts on a nylon mesh or in a sponge such that they deposit extracellular matrix (3,4). The reorganization and contraction of collagen matrix by included fibroblasts also results in a tridimensional dermal layer (5). We adapted the latter method to produce an anchored skin equivalent (ASE) combining the following advantages: i) a large number of similar ASE could be generated with cells cultured from a single skin biopsy. Therefore, this obviates the need for a constant supply of human skin. ii) Replicates could be submitted to quality control during their production. iii) This anchorage design impeded collagen gel contraction thus leading to the production of a SE with a large and defined surface area. iv) The absence of any supporting or embedded material into our ASE facilitated its manipulation and prevented any eventual deleterious effect of foreign matter. Furthermore the absence of any extraneous material avoids an eventual adsorption effect (6).
Document Type: Article de recherche
Issue Date: 1 January 1995
Open Access Date: Restricted access
Document version: VoR
This document was published in: Pharmaceutical Research, Vol. 12 (3), 455-458 (1995)
Plenum Press
Alternative version: 7617537
Collection:Articles publiés dans des revues avec comité de lecture

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