Reduction of caveolin 1 gene expression in lung carcinoma cell lines

Authors: Racine, ClaudiaBélanger, MartinHirabayashi, HirohisaBoucher, MaryléneChakir, JamilaCouët, Jacques
Abstract: Caveolae are plasma membrane microdomains that have been implicated in organizing and concentrating certain signaling molecules. Caveolins, constitute the main structural proteins of caveolae. Caveolae are abundant in terminally differentiated cell types. However, caveolin-1 is down-regulated in transformed cells and may have a potential tumor suppressor activity. In the lung, caveolae are present in the endothelium, smooth muscle cells, fibroblasts as well as in type I pneumocytes. The presence of caveolae and caveolin expression in the bronchial epithelium, although probable, has not been investigated in human. We were interested to see if the bronchial epithelia express caveolins and if this expression was modified in cancer cells. We thus tested for caveolin-1 and -2 expression several bronchial epithelial primary cell lines as well as eight lung cancer cell lines and one larynx tumor cell line. Both caveolin-1 and -2 are expressed in all normal bronchial cell lines. With the exception of Calu-1 cell line, all cancer cell lines showed very low or no expression of caveolin-1 while caveolin-2 expression was similar to the one observed in normal bronchial epithelial cells.
Document Type: Article de recherche
Issue Date: 25 May 2002
Open Access Date: Restricted access
Document version: VoR
This document was published in: Biochemical and Biophysical Research Communications, Vol. 255 (3), 580–586 (1999)
Academic Press
Alternative version: 10.1006/bbrc.1999.0236
Collection:Articles publiés dans des revues avec comité de lecture

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