G2/M blockade by paclitaxel induces caveolin-1 expression in A549 lung cancer cells : caveolin-1 as a marker of cytotoxicity.

Auteur(s): Roussel, ÉliseBélanger, MartinCouët, Jacques
Résumé: Caveolins are highly expressed in terminally differentiated cells, but this expression is down-regulated in various cancer cell lines. Exposure to low doses of paclitaxel (taxol) is sufficient to up-regulate caveolin-1, suggesting that a mild cytotoxic stress induces a response implying caveolin and caveolae. Here we show that this up-regulation is sustained even after the cessation of paclitaxel treatment. After exposure to a cytostatic dose of paclitaxel (50 nM), A549 lung cancer cells are blocked in the G2/M cell cycle phase. After removal of paclitaxel, cell death occurs, accompanied with an increase in caveolin expression, suggesting an effect of caveolin in this process. Three days post-paclitaxel treatment, surviving A549 cells were passaged and only a half of them adhered to the culture dish. Adhering cells (still mainly in the G2/M cell cycle phase) were still unable to grow and progressively entered in an apoptotic state. This study suggests that effects of a low dose of paclitaxel were still present even 1 week after drug removal and that caveolin-1 is a good marker of cytotoxicity.
Type de document: Article de recherche
Date de publication: 1 novembre 2004
Date de la mise en libre accès: 14 novembre 2017
Version du document: AM
Lien permanent: http://hdl.handle.net/20.500.11794/15828
Ce document a été publié dans: Anti-cancer drugs, Vol. 15 (10), 961-967 (2004)
Rapid Communications of Oxford
Autre version disponible: 15514565
Collection :Articles publiés dans des revues avec comité de lecture

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