Isolation and biological characterization of phosphate solubilizing bacteria strains from the rhizospheric soils of corn in Mali

Authors: Traoré, Lamine; Babana, Amadou Hamadoun; Samake, Fassé; Yattara, Inamoud Ibny; Antoun, Hani; Lahbib, Messaoud
Other Title(s): L’isolement et la caractérisation biologique des souches de bactéries du sol rhizosphérique de maïs solubilisant le phosphate au Mali
Abstract: Forty-eight bacteria isolates from the rhizosphere of three cultivars of corn and three different soils were examined for their phosphate solubiling ability. The tests were realized on NBRIP (National Botanical Research Institute of Phosphate Growth medium) containing the Tilemsi phosphate rock (TPR) as the only source of insoluble phosphorus. All the isolates solubilized the TPR in solid and liquid media. Twenty isolates were selected for their high solubilizing capacity in liquid medium (105 to 311 mg P/ ml/g of TPR). These isolates were submitted to different stress conditions (acid medium with pH 7 to 5, five successive growths and the temperature range of 30°C to 45°C). Only six isolates (I 1 , I 2 , I 3 , I 4 I 5 ad I 6) were able to maintain their ability of solubilizing the TPR and also they were not antagonistics. These selected isolates were also tested for their solubilization efficiency (SE) of TPR and phytate on NBRIP solid medium containing the TPR and phytate as the only insoluble source of phosphorus and phytate respectively. The maximum solubilization (300%) for TPR was obtained with the isolate I 5 and 167% for phytate with the isolate I 1 . It has been observed that the bacteria isolates have an average solubilization capacity of 18.54 kg P 2 O 5 out off 30 kg contained in 100 kg of PNT. The same bacteria strains were tested for their plant growth promotion (PGPR) characteristics which indicated that all produced low molecular weight Organic Acids, Siderophores, Indole Acetic Acid (IAA or auxine), but none of them produced the Hydrocyanic Acid (HCN).
Document Type: Article de recherche
Issue Date: 1 January 2012
Open Access Date: 25 October 2017
Document version: VoR
This document was published in: Les Cahiers de l’Economie Rurale, No 12
Collection:Autres articles publiés

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