Active 3'-5' cyclic nucleotide phosphodiesterases are present in detergent-resistant membranes of mural granulosa cells

DC FieldValueLanguage
dc.contributor.authorBergeron, Annick-
dc.contributor.authorGuillemette, Christine-
dc.contributor.authorSirard, Marc-André-
dc.contributor.authorRichard, François-
dc.date.accessioned2016-05-19T20:12:47Z-
dc.date.available10000-01-01-
dc.date.issued2016-01-04-
dc.identifier.issn1031-3613fr_CA
dc.identifier.urihttp://hdl.handle.net/20.500.11794/1568-
dc.description.abstractLipids rafts are specialised membrane microdomains involved in cell signalling that can be isolated as detergent-resistant membranes (DRMs). The second messenger cyclic AMP (cAMP) has a central role in cell signalling in the ovary and its degradation is carried out by the phosphodiesterase (PDE) enzyme family. We hypothesised that PDEs could be functionally present in the lipid rafts of porcine mural granulosa cell membranes. PDE6C, PDE8A and PDE11A were detected by dot blot in the DRMs and the Triton-soluble fraction of the mural granulosa cells membrane and the cytosol. As shown by immunocytochemistry, PDEs showed clear immunostaining in mural granulosa cell membranes and the cytosol. Interestingly, cAMP-PDE activity was 18 times higher in the DRMs than in the Triton-soluble fraction of cell membranes and was 7.7 times higher in the cytosol than in the DRMs. cAMP-PDE activity in mural granulosa cells was mainly contributed by the PDE8 and PDE11 families. This study shows that PDEs from the PDE8 and PDE11 families are present in mural granulosa cells and that the cAMP-PDE activity is mainly contributed by the cytosol. In the cell membrane, the cAMP-PDE activity is mainly contributed by the DRMs. In addition, receptors for prostaglandin E2 and LH, two G-protein-coupled receptors, are present in lipid rafts and absent from the non-raft fraction of the granulosa cell membrane. These results suggest that in these cells, the lipid rafts exist as a cell-signalling platform and PDEs are one of the key enzyme families present in the raft.fr_CA
dc.languageENGfr_CA
dc.publisherCSIROfr_CA
dc.subjectcAMP-PDE activityfr_CA
dc.subjectDRMsfr_CA
dc.subjectPDE8fr_CA
dc.subjectPDE11fr_CA
dc.subjectraftfr_CA
dc.titleActive 3'-5' cyclic nucleotide phosphodiesterases are present in detergent-resistant membranes of mural granulosa cellsfr_CA
dc.typeCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche-
dcterms.bibliographicCitationReproduction, fertility and development, (2016)fr_CA
dc.audienceProfesseurs (Enseignement supérieur)fr_CA
dc.audienceÉtudiantsfr_CA
dc.audienceDoctorantsfr_CA
dc.audienceMicrobiologistesfr_CA
dc.identifier.doi10.1071/RD15243fr_CA
dc.identifier.pubmed26724956fr_CA
dc.subject.rvmGranulosafr_CA
dc.subject.rvmNucléotides cycliques phosphodiestérasesfr_CA
dc.subject.rvmAMP cycliquefr_CA
dc.subject.rvmMicrodomaines membranairesfr_CA
rioxxterms.versionVersion of Recordfr_CA
rioxxterms.version_of_recordhttps://doi.org/10.1071/RD15243.fr_CA
rioxxterms.project.funder_namefqrfr_CA
bul.rights.periodeEmbargoInfinifr_CA
Collection:Articles publiés dans des revues avec comité de lecture

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