Real-time PCR quantification of mycobacterium immunogenum in used metalworking fluids

DC FieldValueLanguage
dc.contributor.authorVeillette, Marc-
dc.contributor.authorPagé, Gabrielle-
dc.contributor.authorThorne, Peter S.-
dc.contributor.authorDuchaine, Caroline-
dc.description.abstractRapid detection and quantification of Mycobacterium immunogenum in field samples of metalworking fluids (MWFs) is important for factory fluid surveillance programs. The applicability of the developed DNA extraction and quantitative real-time PCR (qPCR) methods to detect and quantify M. immunogenum in used MWFs was evaluated. Total DNA from these samples was extracted, and M. immunogenum measured by qPCR by comparison with a standard curve derived from plasmid vectors. PCR counts were compared with bacterial culture counts. PCR counts of M. immunogenum varied from 1.42 × 103 to 3.68 × 106 cells/mL of MWFs. Recovery of M. immunogenum by bacterial culture varied from 2.5% to 70% of qPCR count in corresponding samples. Quantitative PCR could be used to measure M. immunogenum load in MWF samples with greater sensitivity and shorter processing time than the classic bacterial culture-based approach. The proposed qPCR approach could be routinely used in real-time PCR-equipped laboratories to provide early detection of M. immunogenum and to control proliferation that probably leads to hypersensitivity pneumonitis in exposed workers.fr_CA
dc.publisherTaylor and Francisfr_CA
dc.subjectM. immunogenumfr_CA
dc.subjectMetalworking fluidsfr_CA
dc.subjectQuantitative PCRfr_CA
dc.titleReal-time PCR quantification of mycobacterium immunogenum in used metalworking fluidsfr_CA
dc.typeCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche-
dcterms.bibliographicCitationJournal of Occupational and Environmental Hygiene, Vol. 5 (12), 755–760 (2008)fr_CA
dc.audienceProfesseurs (Enseignement supérieur)fr_CA
dc.subject.rvmFluides de coupefr_CA
dc.subject.rvmRéaction en chaîne de la polymérasefr_CA
rioxxterms.versionVersion of Recordfr_CA
Collection:Articles publiés dans des revues avec comité de lecture

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