Real-time PCR quantification of mycobacterium immunogenum in used metalworking fluids

Auteur(s): Veillette, MarcPagé, Gabrielle; Thorne, Peter S.; Duchaine, Caroline
Résumé: Rapid detection and quantification of Mycobacterium immunogenum in field samples of metalworking fluids (MWFs) is important for factory fluid surveillance programs. The applicability of the developed DNA extraction and quantitative real-time PCR (qPCR) methods to detect and quantify M. immunogenum in used MWFs was evaluated. Total DNA from these samples was extracted, and M. immunogenum measured by qPCR by comparison with a standard curve derived from plasmid vectors. PCR counts were compared with bacterial culture counts. PCR counts of M. immunogenum varied from 1.42 × 103 to 3.68 × 106 cells/mL of MWFs. Recovery of M. immunogenum by bacterial culture varied from 2.5% to 70% of qPCR count in corresponding samples. Quantitative PCR could be used to measure M. immunogenum load in MWF samples with greater sensitivity and shorter processing time than the classic bacterial culture-based approach. The proposed qPCR approach could be routinely used in real-time PCR-equipped laboratories to provide early detection of M. immunogenum and to control proliferation that probably leads to hypersensitivity pneumonitis in exposed workers.
Type de document: Article de recherche
Date de publication: 29 octobre 2008
Date de la mise en libre accès: Accès restreint
Version du document: VoR
Lien permanent: http://hdl.handle.net/20.500.11794/12879
Ce document a été publié dans: Journal of Occupational and Environmental Hygiene, Vol. 5 (12), 755–760 (2008)
https://doi.org/10.1080/15459620802446343
Taylor and Francis
Autre version disponible: 10.1080/15459620802446343
18821262
Collection :Articles publiés dans des revues avec comité de lecture

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