Molecular detection of phytophthora ramorum by real-time polymerase chain reaction using taqMan, SYBR green,and molecular beacons

DC FieldValueLanguage
dc.contributor.authorBilodeau, G. J.-
dc.contributor.authorLévesque, André-
dc.contributor.authorCock, A.W.A.M. de (Arthur Wilhelmus Antonius Maria)-
dc.contributor.authorDuchaine, Caroline-
dc.contributor.authorBrière, Stephan-
dc.contributor.authorUribe, P.-
dc.contributor.authorMartin, Frank-
dc.contributor.authorHamelin, Richard C.-
dc.date.accessioned2016-11-15T18:42:41Z-
dc.date.available2016-11-15-
dc.date.issued2006-11-22-
dc.identifier.issn0031-949Xfr_CA
dc.identifier.urihttp://hdl.handle.net/20.500.11794/12161-
dc.description.abstractSudden oak death, caused by Phytophthora ramorum, is a severe disease that affects many species of trees and shrubs. This pathogen is spreading rapidly and quarantine measures are currently in place to prevent dissemination to areas that were previously free of the pathogen. Molecular assays that rapidly detect and identify P. ramorum frequently fail to reliably distinguish between P. ramorum and closely related species. To overcome this problem and to provide additional assays to increase confidence, internal transcribed spacer (ITS), ß-tubulin, and elicitin gene regions were sequenced and searched for polymorphisms in a collection of Phytophthora spp. Three different reporter technologies were compared: molecular beacons, TaqMan, and SYBR Green. The assays differentiated P. ramorum from the 65 species of Phytophthora tested. The assays developed were also used with DNA extracts from 48 infected and uninfected plant samples. All environmental samples from which P. ramorum was isolated by PARP-V8 were detected using all three real-time PCR assays. However, 24% of the samples yielded positive real-time PCR assays but no P. ramorum cultures, but sequence analysis of the coxI and II spacer region confirmed the presence of the pathogen in most samples. The assays based on detection of the ITS and elicitin regions using TaqMan tended to have lower cycle threshold values than those using ß-tubulin and seemed to be more sensitive.fr_CA
dc.languageengfr_CA
dc.publisherAmerican Phytopathological Societyfr_CA
dc.titleMolecular detection of phytophthora ramorum by real-time polymerase chain reaction using taqMan, SYBR green,and molecular beaconsfr_CA
dc.typeCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche-
dcterms.bibliographicCitationPhytopathology, Vol. 97 (5), 632-642 (2007)fr_CA
dc.audienceProfesseurs (Enseignement supérieur)fr_CA
dc.audienceÉtudiantsfr_CA
dc.audienceDoctorantsfr_CA
dc.audienceMicrobiologistesfr_CA
dc.audienceIngénieurs forestiersfr_CA
dc.identifier.doi10.1094/PHYTO-97-5-0632fr_CA
dc.identifier.pubmed18943583fr_CA
dc.subject.rvmPhytophthora ramorum -- Aspect moléculairefr_CA
dc.subject.rvmRéaction en chaîne de la polymérasefr_CA
dc.subject.rvmEncre du chêne rouge -- Préventionfr_CA
rioxxterms.versionVersion of Recordfr_CA
rioxxterms.version_of_recordhttps://doi.org/10.1094/PHYTO-97-5-0632fr_CA
rioxxterms.project.funder_nameautre-
bul.rights.periodeEmbargoInfinifr_CA
Collection:Articles publiés dans des revues avec comité de lecture

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