Publication :
Characterization of cAMP-phosphodiesterase activity in bovine seminal plasma

En cours de chargement...
Vignette d'image
Direction de publication
Direction de recherche
Titre de la revue
ISSN de la revue
Titre du volume
American Society of Andrology
Projets de recherche
Structures organisationnelles
Numéro de revue

The second messenger cyclic adenosine monophosphate (cAMP) has a central role in sperm physiology. Extracellular cAMP can besequentially degraded into 50AMP and adenosine by ecto-phosphodiesterases (ecto-PDE) and ecto-nucleotidases, a phenomenoncalled extracellular cAMP-adenosine pathway. As cAMP-adenosine pathway is involved in sperm capacitation, we hypothesize thatextracellular PDEs are functionally present in seminal plasma. Exclusively measuring cAMP-PDE activity, total activity in bovineseminal plasma was 10.1 1.5 fmoles/min/lg. Using different family-specific PDE inhibitors, we showed that in seminal plasma,the major cAMP-PDE activity was papaverine sensitive (47.5%). These data support the presence of PDE10 in bovine seminal plasmaand was further confirmed by western blot. In epididymal fluid, total cAMP-PDE activity was 48.2 14.8 fmoles/min/lg and weshowed that the major cAMP-PDE activity was 3-isobutyl-methylxanthine insensitive and thus ascribed to PDE8 family. PDE10AmRNAs were found in the testis, epididymis, and seminal vesicles. cAMP-PDE activity is present in bovine seminal plasma and epi-didymal fluid. The results suggest a role for ecto-PDEs present in those fluids in the signaling pathways involved in sperm functions.

Andrology, Vol. 4 (6), 1123-1130 (November 2016)
URL vers la version publiée
cAMP-phosphodiesterase , Seminal plasma , Spermatozoa
Type de document
article de recherche