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Publication :
Gastrointestinal stability and cytotoxicity of bacteriocins from gram-positive and gram-negative bacteria : a comparative in vitro study

ali.license-refhttps://creativecommons.org/licenses/by/4.0fr
ali.license-ref.start-date2022-01-25fr
bul.description.provenanceembre spbarfr
bul.rights.addendumPaiement de frais pour publication en libre accèsfr
bul.rights.dateAccepPubl2022-01-25fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorBiron, Éric
dc.contributor.authorZirah, Séverine
dc.contributor.authorRebuffat, Sylvie
dc.contributor.authorSoltani, Samira
dc.contributor.authorCouture, Frédéric
dc.contributor.authorFliss, Ismaïl
dc.contributor.authorSubirade, Muriel
dc.contributor.authorBoutin, Yvan.
dc.date.accessioned2022-02-17T14:43:38Z
dc.date.available2022-02-17T14:43:38Z
dc.date.issued2022-01-25
dc.description.abstractBacteriocins are receiving increased attention as potent candidates in food preservation and medicine. Although the inhibitory activity of bacteriocins has been studied widely, little is known about their gastrointestinal stability and toxicity toward normal human cell lines. The aim of this study was to evaluate the gastrointestinal stability and activity of microcin J25, pediocin PA-1, bactofencin A and nisin using in vitro models. In addition cytotoxicity and hemolytic activity of these bacteriocins were investigated on human epithelial colorectal adenocarcinoma cells (Caco-2) and rat erythrocytes, respectively. Pediocin PA-1, bactofencin A, and nisin were observed to lose their stability while passing through the gastrointestinal tract, while microcin J25 is only partially degraded. Besides, selected bacteriocins were not toxic to Caco-2 cells, and integrity of cell membrane was observed to remain unaffected in presence of these bacteriocins at concentrations up to 400 μg/mL. In hemolysis study, pediocin PA-1, bactofencin A, and nisin were observed to lyse rat erythrocytes at concentrations higher than 50 μg/mL, while microcin J25 showed no effect on these cells. According to data indicating gastrointestinal degradation and the absence of toxicity of pediocin PA-1, bactofencin A, and microcin J25 they could potentially be used in food or clinical applications.fr
dc.identifier.doi10.3389/fmicb.2021.780355fr
dc.identifier.issn1664-302Xfr
dc.identifier.pubmed35145490fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/72570
dc.languageengfr
dc.publisherFrontiers Mediafr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectBacteriocinsfr
dc.subjectIn vitro digestionfr
dc.subjectCytotoxicityfr
dc.subjectHemolysisfr
dc.subjectFood preservativefr
dc.subject.rvmBactériocinesfr
dc.subject.rvmTractus gastro-intestinalfr
dc.subject.rvmBactéries Gram positivesfr
dc.subject.rvmBactéries Gram négativesfr
dc.subject.rvmToxicité in vitrofr
dc.subject.rvmHémolysefr
dc.titleGastrointestinal stability and cytotoxicity of bacteriocins from gram-positive and gram-negative bacteria : a comparative in vitro studyfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationFrontiers in microbiology, Vol. 12 (2022)fr
dspace.accessstatus.time2024-03-16 18:10:07
dspace.entity.typePublication
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rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.project.funder-nameInternational Development Research Centerfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.3389/fmicb.2021.780355fr

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