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Normal human merkel cells are present in epidermal cell populations isolated and cultured from glabrous and hairy skin sites

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bul.description.provenanceeb spbfr
bul.rights.dateAccepPubl2015-12-08fr
bul.rights.periodeEmbargoforeverfr
bul.rights.raisonEmbargoInfiniPour que le document soit diffusé en libre accès, en accord avec le délai prescrit par l’éditeur de Journal of investigative dermatology, il faudrait déposer la version acceptée pour publication, incluant toutes les modifications demandées, mais sans la mise en page de la revue. Pour ce faire, effectuez une demande de modification à l’aide de la liste des dépôts diffusés à partir du tableau de suivi.fr
bul.rights.typeDatedatePublicationfr
dc.audience.peerreview1fr
dc.contributor.authorCaouette, Louise
dc.contributor.authorGermain, Lucie
dc.contributor.authorLarouche, Danielle
dc.contributor.authorCouture, Véronique
dc.contributor.authorFugère, Claudia.
dc.contributor.authorGuignard, Rina
dc.contributor.authorFradette, Julie
dc.contributor.authorRoy, Alphonse
dc.contributor.authorBeauparlant, Annie.
dc.date.accessioned2018-01-26T15:19:40Z
dc.date.available9999-12-31
dc.date.issued2015-12-08
dc.description.abstractThe Merkel cell is a highly specialized cell that primarily acts as a slowly adapting mechanoreceptor. Merkel cells are scarce in normal skin but can be identified by the expression of distinct keratin filaments. Merkel cells constitute a very unique population and many questions still remain as to their origin, number, proliferative capacity, and functions in cutaneous biology. The dissociation of epidermal cells from skin is a widely used technique to extract and culture keratinocytes. We took advantage of a two-step extraction method to quantify keratin-20-expressing Merkel cells among total cutaneous cells obtained from either hairy or glabrous skin biopsies. Flow cytometry analysis revealed that keratin-20-labeled Merkel cells represent between 3.6% and 5.7% of freshly dissociated basal epidermal cells. No significant differences were seen between samples derived from glabrous palmar and hairy anatomic sites, from children and adult, respectively. We also report on the presence of Merkel cells in primary and first subcultures of epidermal cells indicating their capacity to remain viable after extraction from skin of various anatomic sites. To our knowledge, this is the first demonstration of nontumorigenic human Merkel cells in culture in vitro. The persistence of a small number of Merkel cells in culture suggests that, with the development of appropriate culture conditions, these cells could be amplified and further studied to unravel long-standing questions relative to their paracrine function or epithelial origin.fr
dc.identifier.doi10.1046/j.1523-1747.2003.12024.xfr
dc.identifier.issn0022-202Xfr
dc.identifier.pubmed12542538fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/16683
dc.languageengfr
dc.publisherElsevier Sciencefr
dc.rightshttp://purl.org/coar/access_right/c_16ec
dc.subjectCulturefr
dc.subjectHuman skinfr
dc.subjectKeratinsfr
dc.subjectmechanoreceptorfr
dc.subjectMerkel cellsfr
dc.subject.rvmMerkel, Cellules defr
dc.subject.rvmKératinefr
dc.subject.rvmPeaufr
dc.subject.rvmÉpidermefr
dc.subject.rvmCellules humaines -- Culturefr
dc.subject.rvmMécanorécepteursfr
dc.titleNormal human merkel cells are present in epidermal cell populations isolated and cultured from glabrous and hairy skin sitesfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationJournal of Investigative Dermatology, Vol. 120 (2), 313-317 (2003)fr
dspace.accessstatus.time2023-05-26 18:09:32
dspace.entity.typePublication
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rioxxterms.projectMT-12087fr
rioxxterms.project.funder-nameMedical Research Council Canadafr
rioxxterms.project.funder-nameCanadian Institutes of Health Researchfr
rioxxterms.project.funder-nameFonds de Recherche du Québec - Santéfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.1046/j.1523-1747.2003.12024.xfr
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