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Publication :
Pro-angiogenic capacities of microvesicles produced by skin wound myofibroblasts

bul.description.provenancenagfr
bul.rights.dateAccepPubl2017-04-08fr
bul.rights.periodeEmbargoP1Yfr
bul.rights.typeDatedatePublicationfr
dc.audienceMicrobiologistesfr
dc.audienceMédecinsfr
dc.audienceProfesseurs (Enseignement supérieur)fr
dc.audienceDoctorantsfr
dc.audienceÉtudiantsfr
dc.audience.peerreview1fr
dc.contributor.authorBeaudoin-Cloutier, Chanel
dc.contributor.authorMerjaneh, Mays
dc.contributor.authorLanglois, Amélie
dc.contributor.authorLarochelle, Sébastien
dc.contributor.authorRicard-Blum, Sylvie
dc.contributor.authorMoulin, Véronique
dc.date.accessioned2017-06-07T18:17:44Z
dc.date.available2018-04-08T04:00:00Z
dc.date.issued2017-04-08
dc.description.abstractWound healing is a very highly organized process where numerous cell types are tightly regulated to restore injured tissue. Myofibroblasts are cells that produce new extracellular matrix and contract wound edges. We previously reported that the human myofibroblasts isolated from normal wound (WMyos) produced microvesicles (MVs) in the presence of the serum. In this study, MVs were further characterized using a proteomic strategy and potential functions of the MVs were determined. MV proteins isolated from six WMyo populations were separated using two-dimensional differential gel electrophoresis. Highly conserved spots were selected and analyzed using mass spectrometry resulting in the identification of 381 different human proteins. Using the DAVID database, clusters of proteins involved in cell motion, apoptosis and adhesion, but also in extracellular matrix production (21 proteins, enrichment score: 3.32) and in blood vessel development/angiogenesis (19 proteins, enrichment score: 2.66) were identified. Another analysis using the functional enrichment analysis tool FunRich was consistent with these results. While the action of the myofibroblasts on extracellular matrix formation is well known, their angiogenic potential is less studied. To further characterize the angiogenic activity of the MVs, they were added to cultured microvascular endothelial cells to evaluate their influence on cell growth and migration using scratch test and capillary-like structure formation in Matrigel®. The addition of a MV-enriched preparation significantly increased endothelial cell growth, migration and capillary formation compared with controls. The release of microvesicles by the wound myofibroblasts brings new perspectives to the field of communication between cells during the normal healing process.fr
dc.identifier.doi10.1007/s10456-017-9554-9fr
dc.identifier.issn1573-7209fr
dc.identifier.pubmed28391377fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/14301
dc.languageengfr
dc.publisherKluwerfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectAngiogenesisfr
dc.subjectEndothelial cellsfr
dc.subjectHealingfr
dc.subjectHumanfr
dc.subjectMicrovesiclefr
dc.subjectMyofibroblastfr
dc.subjectProteomicfr
dc.subjectSkinfr
dc.subject.rvmMyofibroblastesfr
dc.subject.rvmFacteurs angiogéniquesfr
dc.subject.rvmExosomesfr
dc.subject.rvmPeau -- Lésions et blessuresfr
dc.titlePro-angiogenic capacities of microvesicles produced by skin wound myofibroblastsfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationAngiogenesis, Vol. 22, 1-14 (2017)fr
dspace.accessstatus.time2024-03-16 18:01:40
dspace.entity.typePublication
relation.isAuthorOfPublication019e2498-6028-4f04-87d5-e755bed3712c
relation.isAuthorOfPublication03cf251f-a157-4566-98d1-c486cbe08ff2
relation.isAuthorOfPublication2d1affad-ac80-476b-8469-736808ba1ce4
relation.isAuthorOfPublication3f4cf214-dc4c-42f4-853c-0f6f7650d95d
relation.isAuthorOfPublicatione60c26f0-355c-4a22-9655-7cd22caf2391
relation.isAuthorOfPublication.latestForDiscovery019e2498-6028-4f04-87d5-e755bed3712c
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.project.funder-nameFonds de Recherche du Québec - Santéfr
rioxxterms.versionAccepted Manuscript (AM)fr
rioxxterms.version-of-recordhttps://doi.org/10.1007/s10456-017-9554-9fr

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