Publication : Pro-angiogenic capacities of microvesicles produced by skin wound myofibroblasts
bul.description.provenance | nag | fr |
bul.rights.dateAccepPubl | 2017-04-08 | fr |
bul.rights.periodeEmbargo | P1Y | fr |
bul.rights.typeDate | datePublication | fr |
dc.audience | Microbiologistes | fr |
dc.audience | Médecins | fr |
dc.audience | Professeurs (Enseignement supérieur) | fr |
dc.audience | Doctorants | fr |
dc.audience | Étudiants | fr |
dc.audience.peerreview | 1 | fr |
dc.contributor.author | Beaudoin-Cloutier, Chanel | |
dc.contributor.author | Merjaneh, Mays | |
dc.contributor.author | Langlois, Amélie | |
dc.contributor.author | Larochelle, Sébastien | |
dc.contributor.author | Ricard-Blum, Sylvie | |
dc.contributor.author | Moulin, Véronique | |
dc.date.accessioned | 2017-06-07T18:17:44Z | |
dc.date.available | 2018-04-08T04:00:00Z | |
dc.date.issued | 2017-04-08 | |
dc.description.abstract | Wound healing is a very highly organized process where numerous cell types are tightly regulated to restore injured tissue. Myofibroblasts are cells that produce new extracellular matrix and contract wound edges. We previously reported that the human myofibroblasts isolated from normal wound (WMyos) produced microvesicles (MVs) in the presence of the serum. In this study, MVs were further characterized using a proteomic strategy and potential functions of the MVs were determined. MV proteins isolated from six WMyo populations were separated using two-dimensional differential gel electrophoresis. Highly conserved spots were selected and analyzed using mass spectrometry resulting in the identification of 381 different human proteins. Using the DAVID database, clusters of proteins involved in cell motion, apoptosis and adhesion, but also in extracellular matrix production (21 proteins, enrichment score: 3.32) and in blood vessel development/angiogenesis (19 proteins, enrichment score: 2.66) were identified. Another analysis using the functional enrichment analysis tool FunRich was consistent with these results. While the action of the myofibroblasts on extracellular matrix formation is well known, their angiogenic potential is less studied. To further characterize the angiogenic activity of the MVs, they were added to cultured microvascular endothelial cells to evaluate their influence on cell growth and migration using scratch test and capillary-like structure formation in Matrigel®. The addition of a MV-enriched preparation significantly increased endothelial cell growth, migration and capillary formation compared with controls. The release of microvesicles by the wound myofibroblasts brings new perspectives to the field of communication between cells during the normal healing process. | fr |
dc.identifier.doi | 10.1007/s10456-017-9554-9 | fr |
dc.identifier.issn | 1573-7209 | fr |
dc.identifier.pubmed | 28391377 | fr |
dc.identifier.uri | http://hdl.handle.net/20.500.11794/14301 | |
dc.language | eng | fr |
dc.publisher | Kluwer | fr |
dc.rights | http://purl.org/coar/access_right/c_abf2 | |
dc.subject | Angiogenesis | fr |
dc.subject | Endothelial cells | fr |
dc.subject | Healing | fr |
dc.subject | Human | fr |
dc.subject | Microvesicle | fr |
dc.subject | Myofibroblast | fr |
dc.subject | Proteomic | fr |
dc.subject | Skin | fr |
dc.subject.rvm | Myofibroblastes | fr |
dc.subject.rvm | Facteurs angiogéniques | fr |
dc.subject.rvm | Exosomes | fr |
dc.subject.rvm | Peau -- Lésions et blessures | fr |
dc.title | Pro-angiogenic capacities of microvesicles produced by skin wound myofibroblasts | fr |
dc.type | article de recherche | |
dc.type.legacy | COAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche | fr |
dcterms.bibliographicCitation | Angiogenesis, Vol. 22, 1-14 (2017) | fr |
dspace.accessstatus.time | 2024-03-16 18:01:40 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 019e2498-6028-4f04-87d5-e755bed3712c | |
relation.isAuthorOfPublication | 03cf251f-a157-4566-98d1-c486cbe08ff2 | |
relation.isAuthorOfPublication | 2d1affad-ac80-476b-8469-736808ba1ce4 | |
relation.isAuthorOfPublication | 3f4cf214-dc4c-42f4-853c-0f6f7650d95d | |
relation.isAuthorOfPublication | e60c26f0-355c-4a22-9655-7cd22caf2391 | |
relation.isAuthorOfPublication.latestForDiscovery | 019e2498-6028-4f04-87d5-e755bed3712c | |
relation.isResourceTypeOfPublication | 4c433ef5-3937-4530-8252-cca17d715747 | |
relation.isResourceTypeOfPublication.latestForDiscovery | 4c433ef5-3937-4530-8252-cca17d715747 | |
rioxxterms.project.funder-name | Natural Sciences and Engineering Research Council of Canada | fr |
rioxxterms.project.funder-name | Fonds de Recherche du Québec - Santé | fr |
rioxxterms.version | Accepted Manuscript (AM) | fr |
rioxxterms.version-of-record | https://doi.org/10.1007/s10456-017-9554-9 | fr |
Fichiers
Bundle original
1 - 4 sur 4
En cours de chargement...
- Nom :
- AGEN-D-16-00153_R3.pdf
- Taille :
- 1.46 MB
- Format :
- Adobe Portable Document Format
En cours de chargement...
- Nom :
- Figures.pdf
- Taille :
- 955.29 KB
- Format :
- Adobe Portable Document Format
En cours de chargement...
- Nom :
- Table 1S.pdf
- Taille :
- 98.27 KB
- Format :
- Adobe Portable Document Format
En cours de chargement...
- Nom :
- Table 2S annexe liste cluster.pdf
- Taille :
- 4.4 MB
- Format :
- Adobe Portable Document Format