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Resistance of aerosolized bacterial viruses to four germicidal products

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ali.license-refhttps://creativecommons.org/licenses/by/4.0fr
ali.license-ref.start-date2020-03-30fr
bul.description.provenancelf spbfr
bul.rights.dateAccepPubl2016-12-28fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorDuchaine, Caroline
dc.contributor.authorMichel, Kevin
dc.contributor.authorHa, Thi-Lan
dc.contributor.authorMoineau, Sylvain
dc.contributor.authorRobine, Enric
dc.contributor.authorTurgeon, Nathalie
dc.date.accessioned2020-04-21T14:05:12Z
dc.date.available2020-04-21T14:05:12Z
dc.date.issued2016-12-28
dc.description.abstractViral diseases can spread through a variety of routes including aerosols. Yet, limited data are available on the efficacy of aerosolized chemicals to reduce viral loads in the air. Bacteriophages (phages) are often used as surrogates for hazardous viruses in aerosol studies because they are inexpensive, easy to handle, and safe for laboratory workers. Moreover, several of these bacterial viruses display physical characteristics similar to pathogenic human and animal viruses, like morphological size, type of nucleic acids, capsid morphology, and the presence of an envelope. In this study, the efficacy of four chemicals was evaluated on four airborne phages at two different relative humidity levels. Non-tailed bacteriophages MS2 (single-stranded RNA), ϕ6 (double-stranded RNA, enveloped), PR772 (double-stranded DNA), and ϕX174 (single-stranded DNA) were first aerosolized in a 55L rotative environmental chamber at 19°C with 25% and 50% relative humidity. Then, hydrogen peroxide, Eugenol (phenylpropene used in commercial perfumes and flavorings), Mist® (automobile disinfectant containing Triethylene glycol), and Pledge® (multisurface disinfectant containing Isopropanol, n-Alkyl Dimethyl Benzyl Amonium Chlorides, and n-Alkyl Dimethyl Ethylbenzyl Ammonium Chloride) were nebulized with the phages using a separate nebulizer. Aerosols were maintained in suspension during 10 minutes, 1 hour, and 2 hours. Viral aerosols were sampled using an SKC BioSampler and samples were analyzed using qPCR and plaque assays. The resistance levels of the four phages varied depending on the relative humidity (RH) and germicidal products tested. Phage MS2 was the most stable airborne virus under the environmental conditions tested while phage PR772 was the least stable. Pledge® and Eugenol reduced the infectivity of all airborne phages tested. At 25% RH, Pledge® and Eugenol were more effective at reducing infectivity of RNA phages ϕ6 and MS2. At 50% RH, Pledge® was the most effective agent against phage MS2. These findings illustrate that various airborne viruses should be tested to demonstrate the effectiveness of germicidal treatments. This research also provides a set of parameters for testing germicidal products in large-scale settings to reduce the risk of virus transmission.fr
dc.identifier.doi10.1371/journal.pone.0168815fr
dc.identifier.issn1932-6203fr
dc.identifier.pubmed5193356fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/38875
dc.languageengfr
dc.publisherPublic Library of Sciencefr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.rvmAérosols -- Microbiologiefr
dc.subject.rvmBactériophagesfr
dc.subject.rvmAntiseptiquesfr
dc.titleResistance of aerosolized bacterial viruses to four germicidal productsfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationPLoS One, Vol. 11 (12), 1-14 (2016)fr
dspace.accessstatus.time2023-05-25 18:28:44
dspace.entity.typePublication
relation.isAuthorOfPublication002082bc-ec48-49a0-9132-31a2b1244bee
relation.isAuthorOfPublication02f4258f-0e57-4a18-a277-0da5b65887fb
relation.isAuthorOfPublication599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isAuthorOfPublicationb04f482c-1dc3-4fa8-9592-d3c649f8374f
relation.isAuthorOfPublication.latestForDiscovery002082bc-ec48-49a0-9132-31a2b1244bee
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.project.funder-nameMinistère de l'Écologie, du Développement Durable et de l'Énergiefr
rioxxterms.project.funder-nameFrench research program PRIMEQUAL. Convention n°12-MRES-Primequal-5-CVS-37fr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.1371/journal.pone.0168815fr
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