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Mechanical strain induces the production of spheroid mineralized microparticles in the aortic valve through a RhoA/ROCK-dependent mechanism.

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bul.description.provenancesdifr_CA
bul.description.provenancespbfr_CA
bul.rights.dateAccepPubl2013-12-22fr_CA
bul.rights.periodeEmbargoforeverfr_CA
bul.rights.raisonEmbargoInfiniPour que le document soit diffusé en libre accès, en accord avec le délai prescrit par l’éditeur de Journal of molecular and cellular cardiology, il faudrait déposer la version acceptée par le comité de lecture, incluant toutes les corrections, sans la mise en page de la revue. Pour ce faire, effectuez une demande de modification à l’aide de la liste des dépôts diffusés à partir du tableau de suivi.fr_CA
bul.rights.typeDatedatePublicationfr_CA
dc.audienceProfesseurs (Enseignement supérieur)fr_CA
dc.audienceÉtudiantsfr_CA
dc.audienceDoctorantsfr_CA
dc.audienceCardiologuesfr_CA
dc.audienceMédecinsfr_CA
dc.contributor.authorBouchareb, Rihab
dc.contributor.authorFournier, Dominique
dc.contributor.authorBoulanger, Marie-Chloé
dc.contributor.authorMessaddeq, Younès
dc.contributor.authorPibarot, Philippe
dc.contributor.authorMathieu, Patrick
dc.date.accessioned2016-06-22T17:46:07Z
dc.date.available9999-12-31
dc.date.issued2013-12-22
dc.description.abstractCalcific aortic valve disease (CAVD) is a chronic disorder characterized by an abnormal mineralization of the leaflets, which is accelerated in bicuspid aortic valve (BAV). It is suspected that mechanical strain may promote/enhance mineralization of the aortic valve. However, the effect of mechanical strain and the involved pathways during mineralization of the aortic valve remains largely unknown. Valve interstitial cells (VICs) were isolated and studied under strain conditions. Human bicuspid aortic valves were examined as a model relevant to increase mechanical strain. Cyclic strain increased mineralization of VICs by several-fold. Scanning electron microscope (SEM) and energy dispersive X-ray (EDX) analyses revealed that mechanical strain promoted the formation of mineralized spheroid microparticles, which coalesced into larger structure at the surface of apoptotic VICs. Apoptosis and mineralization were closely associated with expression of ENPP1. Inhibition of ENPP1 greatly reduced mineralization of VIC cultures. Through several lines of evidence we showed that mechanical strain promoted the export of ENPP1-containing vesicles to the plasma membrane through a RhoA/ROCK pathway. Studies conducted in human BAV revealed the presence of spheroid mineralized structures along with the expression of ENPP1 in areas of high mechanical strain. Mechanical strain promotes the production and accumulation of spheroid mineralized microparticles by VICs, which may represent one important underlying mechanism involved in aortic valve mineralization. RhoA/ROCK-mediated export of ENPP1 to the plasma membrane promotes strain-induced mineralization of VICs.fr_CA
dc.identifier.doi10.1016/j.yjmcc.2013.12.009fr_CA
dc.identifier.issn0022-2828fr_CA
dc.identifier.pubmed24368096fr_CA
dc.identifier.urihttp://hdl.handle.net/20.500.11794/7188
dc.languageengfr_CA
dc.publisherAcademic Press Inc, Ltd.fr_CA
dc.rightshttp://purl.org/coar/access_right/c_16ec
dc.subjectCalcific aortic valve diseasefr_CA
dc.subjectENPP1fr_CA
dc.subjectMineralized microparticlesfr_CA
dc.subjectRhoAfr_CA
dc.subjectStrainfr_CA
dc.subjectValve interstitial cellsfr_CA
dc.subject.rvmValvule semi-lunaire de l'ostium aortique -- Calcificationfr_CA
dc.subject.rvmBicuspidie valvulaire aortiquefr_CA
dc.subject.rvmMicroparticules membranairesfr_CA
dc.titleMechanical strain induces the production of spheroid mineralized microparticles in the aortic valve through a RhoA/ROCK-dependent mechanism.fr_CA
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche
dcterms.bibliographicCitationJournal of molecular and cellular cardiology, Vol. 67, 49-59 (2014)fr_CA
dspace.accessstatus.time2023-05-25 18:17:07
dspace.entity.typePublication
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rioxxterms.project.funder-nameautrefr_CA
rioxxterms.versionVersion of Record (VoR)fr_CA
rioxxterms.version-of-recordhttps://doi.org/10.1016/j.yjmcc.2013.12.009fr_CA
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