Publication : A convenient approach to prepare topologically segregated bilayer beads for one-bead two-compound combinatorial peptide libraries
bul.description.provenance | cl | fr_CA |
bul.rights.dateAccepPubl | 2012-07-18 | fr_CA |
bul.rights.periodeEmbargo | forever | fr_CA |
bul.rights.raisonEmbargoInfini | L’éditeur de International Journal of Peptide Research and Therapeutics n’autorise pas la diffusion dans un dépôt institutionnel de la version déposée. Par contre, il autorise la diffusion de la version acceptée par le comité de lecture, incluant toutes les corrections, avant la mise en page de la revue. Si vous souhaitez que le document soit diffusé en libre accès, en accord avec le délai prescrit par l’éditeur de International Journal of Peptide Research and Therapeutics, il faudrait déposer la version mentionnée ci-dessus. Pour ce faire, effectuez une demande de modification à partir de la liste des dépôts diffusés. Vous pouvez y accéder à partir de votre tableau de suivi. | fr_CA |
bul.rights.typeDate | datePublication | fr_CA |
dc.audience | Professeurs (Enseignement supérieur) | fr_CA |
dc.audience | Doctorants | fr_CA |
dc.audience | Étudiants | fr_CA |
dc.audience | Biochimistes | fr_CA |
dc.audience | Chimistes | fr_CA |
dc.contributor.author | Girard, Anick | |
dc.contributor.author | Biron, Éric | |
dc.contributor.author | Bédard, François | |
dc.date.accessioned | 2016-03-14T18:21:25Z | fr_CA |
dc.date.available | 9999-12-31 | fr_CA |
dc.date.issued | 2012-07-18 | fr_CA |
dc.description.abstract | One-bead one-compound (OBOC) combinatorial peptide libraries have been used to identify ligands and modulators for a wide variety of biological targets. While being very efficient with linear peptides, OBOC libraries with N-terminally blocked peptides or with unsequenceable building blocks require encoding. To fully exploit OBOC combinatorial methods with cyclic peptides and peptidomimetics, topologically segregated bilayer beads have been developed. This strategy offers the opportunity to synthesize two compounds per bead, i.e. with one compound exposed on the bead surface for screening, and the other one found within the inner layer as a tag for sequencing and compound identification. Bead segregation often involves the use of unstable derivatives or requires a series of protection–deprotection steps. In order to expedite and optimize bead segregation, the performance of various reagents has been studied. The results obtained herein show that bead segregation can be efficiently performed with commercially available reagents. Finally, in order to control outer/inner layer ratios in segregated beads, the effects of different parameters have been evaluated. We report a straightforward and efficient procedure to prepare topologically segregated bilayer beads in a wide range of controllable, predictable, and reproducible outer versus inner ratios. | en |
dc.identifier.doi | 10.1007/s10989-012-9316-x | fr_CA |
dc.identifier.issn | 1573-3149 | fr_CA |
dc.identifier.uri | http://hdl.handle.net/20.500.11794/198 | |
dc.language | eng | en |
dc.publisher | Springer | fr_CA |
dc.rights | http://purl.org/coar/access_right/c_16ec | |
dc.subject.rvm | Chimie combinatoire | fr_CA |
dc.subject.rvm | Cyclopeptides | fr_CA |
dc.subject.rvm | Synthèse en phase solide | fr_CA |
dc.title | A convenient approach to prepare topologically segregated bilayer beads for one-bead two-compound combinatorial peptide libraries | fr_CA |
dc.type | article de recherche | |
dc.type.legacy | COAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche | |
dcterms.bibliographicCitation | International Journal of Peptide Research and Therapeutics, Vol. 19 (1), 13–23 (2013) | fr_CA |
dspace.accessstatus.time | 2024-03-25 18:07:01 | |
dspace.entity.type | Publication | |
relation.isAuthorOfPublication | 07d09997-6da0-41bb-82a2-47efd36b9083 | |
relation.isAuthorOfPublication | 18b333cd-28ac-4a91-b46a-01e33876ce31 | |
relation.isAuthorOfPublication | a4985e3b-8b2e-44b4-aa8d-a0f6b89d602e | |
relation.isAuthorOfPublication.latestForDiscovery | 07d09997-6da0-41bb-82a2-47efd36b9083 | |
relation.isResourceTypeOfPublication | 4c433ef5-3937-4530-8252-cca17d715747 | |
relation.isResourceTypeOfPublication.latestForDiscovery | 4c433ef5-3937-4530-8252-cca17d715747 | |
rioxxterms.project.funder-name | autre | fr_CA |
rioxxterms.version | Version of Record (VoR) | fr_CA |
rioxxterms.version-of-record | https://doi.org/10.1007/s10989-012-9316-x | fr_CA |
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