Publication :
Programming native CRISPR arrays for the generation of targeted immunity

Notice brève
ali.license-refhttps://creativecommons.org/licenses/by/4.0fr
ali.license-ref.start-date2020-03-30fr
bul.description.provenancelf spbfr
bul.rights.dateAccepPubl2016-05-03fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorMoineau, Sylvain
dc.contributor.authorHynes, Alexander
dc.contributor.authorLabrie, Simon
dc.date.accessioned2020-04-17T13:20:59Z
dc.date.available2020-04-17T13:20:59Z
dc.date.issued2016-05-03
dc.description.abstractABSTRACT : The adaptive immune system of prokaryotes, called CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated genes), results in specific cleavage of invading nucleic acid sequences recognized by the cell’s “memory” of past encounters. Here, we exploited the properties of native CRISPR-Cas systems to program the natural “memorization” process, efficiently generating immunity not only to a bacteriophage or plasmid but to any specifically chosen DNA sequence. IMPORTANCE : CRISPR-Cas systems have entered the public consciousness as genome editing tools due to their readily programmable nature. In industrial settings, natural CRISPR-Cas immunity is already exploited to generate strains resistant to potentially disruptive viruses. However, the natural process by which bacteria acquire new target specificities (adaptation) is difficult to study and manipulate. The target against which immunity is conferred is selected stochastically. By biasing the immunization process, we offer a means to generate customized immunity, as well as provide a new tool to study adaptation.fr
dc.identifier.doi10.1128/mBio.00202-16fr
dc.identifier.issn2150-7511fr
dc.identifier.pubmed27143383fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/38830
dc.languageengfr
dc.publisherAmerican Society for Microbiologyfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.rvmSystèmes CRISPR-Casfr
dc.titleProgramming native CRISPR arrays for the generation of targeted immunityfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationmBio, Vol. 7 (3), 1-4 (2016)fr
dspace.accessstatus.time2023-05-25 18:09:37
dspace.entity.typePublication
relation.isAuthorOfPublication599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isAuthorOfPublication5e457313-89bc-4fb2-853b-25175ea6fe35
relation.isAuthorOfPublication7f91fb6e-66c3-4400-a44f-dd4d227ee732
relation.isAuthorOfPublication.latestForDiscovery599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.project.funder-nameTier 1 Canada Research Chair in Bacteriophagesfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.1128/mBio.00202-16fr
Fichiers
Bundle original
Voici les éléments 1 - 1 sur 1
Vignette d'image
Nom :
mBio-2016-Hynes-e00202-16.full.pdf
Taille :
773.88 KB
Format :
Adobe Portable Document Format
Description :
Télécharger
Collections
Articles publiés dans des revues avec comité de lecture