Publication :
Expression of C4.4A in an in vitro human tissue-engineered skin model

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bul.description.provenancelf spbfr
bul.rights.dateAccepPubl2017-09-07fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.audience.peerreview1fr
dc.contributor.authorGermain, Lucie
dc.contributor.authorLarouche, Danielle
dc.contributor.authorRochette-Drouin, Olivier
dc.contributor.authorPloug, Michael
dc.contributor.authorJacobsen, Benedikte
dc.date.accessioned2018-02-02T18:35:07Z
dc.date.available2018-02-02T18:35:07Z
dc.date.issued2017-09-07
dc.description.abstractA multi-LU-domain-containing protein denoted C4.4A exhibits a tightly regulated membrane-associated expression in the suprabasal layers of stratified squamous epithelia such as skin and the esophagus, and the expression of C4.4A is dysregulated in various pathological conditions. However, the biological function of C4.4A remains unknown. To enable further studies, we evaluated the expression of C4.4A in monolayer cultures of normal human keratinocytes and in tissue-engineered skin substitutes (TESs) produced by the self-assembly approach, which allow the formation of a fully differentiated epidermis tissue. Results showed that, in monolayer, C4.4A was highly expressed in the centre of keratinocyte colonies at cell-cell contacts areas, while some cells located at the periphery presented little C4.4A expression. In TES, emergence of C4.4A expression coincided with the formation of the stratum spinosum. After the creation of a wound within the TES, C4.4A expression was observed in the suprabasal keratinocytes of the migrating epithelium, with the exception of the foremost leading keratinocytes, which were negative for C4.4A. Our results are consistent with previous data in mouse embryogenesis and wound healing. Based on these findings, we conclude that this human TES model provides an excellent surrogate for studies of C4.4A and Haldisin expressions in human stratified epithelia.fr
dc.identifier.doi10.1155/2017/2403072fr
dc.identifier.issn2314-6133fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/16850
dc.languageengfr
dc.publisherHindawifr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.rvmPeau artificiellefr
dc.subject.rvmÉpithéliumfr
dc.subject.rvmKératinocytesfr
dc.subject.rvmPeau -- Cultures et milieux de culturefr
dc.titleExpression of C4.4A in an in vitro human tissue-engineered skin modelfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationBioMed Research International, Vol. 2017, 1-9 (2017)fr
dspace.accessstatus.time2023-05-25 18:08:26
dspace.entity.typePublication
relation.isAuthorOfPublication375ce2c4-1171-4ecc-9901-7b68e028f911
relation.isAuthorOfPublication4559dc8d-fb9e-4e20-8117-d4999e7a1c52
relation.isAuthorOfPublicationd904633f-38a1-45de-8e31-63603ffa08cf
relation.isAuthorOfPublicationda1095d5-af23-40a7-9fa8-8020a27a739b
relation.isAuthorOfPublication.latestForDiscovery375ce2c4-1171-4ecc-9901-7b68e028f911
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rioxxterms.project.funder-nameFondation des Pompiers du Québec pour les Grandsfr
rioxxterms.project.funder-nameFonds de Recherche du Québec - Santéfr
rioxxterms.project.funder-nameRéseau de Thérapie Cellulaire et Tissulaire du FRQS (ThéCell)fr
rioxxterms.project.funder-nameCanadian Institutes of Health Researchfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.1155/2017/2403072fr
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