Publication :
Mitochondrial sub-cellular localization of cAMP-specific phosphodiesterase 8A in ovarian follicular cells

Notice brève
ali.license-refhttps://creativecommons.org/licenses/by/4.0fr
ali.license-ref.start-date2020-03-23fr
bul.description.provenanceec spbfr
bul.rights.dateAccepPubl2019-08-28fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorTremblay, Marie-Ève
dc.contributor.authorVernoux, Nathalie
dc.contributor.authorLounas, Amel
dc.contributor.authorRichard, François J.
dc.contributor.authorGermain, Marc
dc.date.accessioned2020-03-27T18:37:05Z
dc.date.available2020-03-27T18:37:05Z
dc.date.issued2019-08-28
dc.description.abstractCyclic adenosine monophosphate (cAMP) is a ubiquitous secondary messenger that plays a central role in endocrine tissue function, particularly in the synthesis of steroid hormones. The intracellular concentration of cAMP is regulated through its synthesis by cyclases and its degradation by cyclic nucleotide phosphodiesterases (PDEs). Although the expression and activity of PDEs impact the specificity and the amplitude of the cAMP response, it is becoming increasingly clear that the sub-cellular localization of PDE emphasizes the spatial regulation of the cell signalling processes that are essential for normal cellular function. We first examined the expression of PDE8A in porcine ovarian cells. PDE8A is expressed in granulosa cells, cumulus cells and oocytes. Second, we assessed the mitochondrial sub-cellular localization of PDE8A. Using western blotting with isolated mitochondrial fractions from granulosa cells and cumulus-oocyte complexes revealed immuno-reactive bands. PDE assay of isolated mitochondrial fractions from granulosa cells measured specific PDE8 cAMP-PDE activity as PF-04957325-sensitive. The immune-reactive PDE8A signal and MitoTracker labelling co-localized supporting mitochondrial sub-cellular localization of PDE8A, which was confirmed using immuno-electron microscopy. Finally, the effect of PDE8 on progesterone production was assessed during the in-vitro maturation of cumulus-oocyte complexes. Using PF-04957325, we observed a significant increase (P < 0.05) in progesterone secretion with follicle-stimulating hormone (FSH). Active mitochondria stained with MitoTracker orange CMTMRos were also increased by the specific PDE8 inhibitor supporting its functional regulation. In conclusion, we propose the occurrence of mitochondrial sub-cellular localization of PDE8A in porcine granulosa cells and cumulus cells. This suggests that there is potential for new strategies for ovarian stimulation and artificial reproductive technologies, as well as the possibility for using new media to improve the quality of oocytes.fr
dc.identifier.doi10.1038/s41598-019-48886-8fr
dc.identifier.issn2045-2322fr
dc.identifier.pubmed31462694fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/38411
dc.languageengfr
dc.publisherNature Publishing Groupfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.rvmAMP cycliquefr
dc.subject.rvmNucléotides cycliques phosphodiestérasesfr
dc.subject.rvmGranulosafr
dc.subject.rvmCumulus (Anatomie)fr
dc.subject.rvmMitochondriesfr
dc.titleMitochondrial sub-cellular localization of cAMP-specific phosphodiesterase 8A in ovarian follicular cellsfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationScientific Reports, Vol. 9 (1), 2019fr
dspace.accessstatus.time2023-09-18 18:15:04
dspace.entity.typePublication
relation.isAuthorOfPublication0c2232f4-8ac9-404d-9f2d-29e845c33cfd
relation.isAuthorOfPublication0cba6f03-3a5c-4d0e-bf4d-fc0036946c81
relation.isAuthorOfPublication6da2e3bd-4847-4e9f-b62b-297d8deecedd
relation.isAuthorOfPublication8367ef55-3028-4735-a279-764d526ea830
relation.isAuthorOfPublication8875aed7-b4bb-4def-a2b7-61d17cbd2825
relation.isAuthorOfPublication.latestForDiscovery0c2232f4-8ac9-404d-9f2d-29e845c33cfd
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.projectNSERC : RGPIN-2014-04774fr
rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.project.funder-nameFonds de Recherche du Québec - Nature et Technologiesfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.1038/s41598-019-48886-8fr
Fichiers
Bundle original
Voici les éléments 1 - 1 sur 1
Vignette d'image
Nom :
Lounas et al. - 2019 - Mitochondrial sub-cellular localization of cAMP-sp.pdf
Taille :
1.62 MB
Format :
Adobe Portable Document Format
Télécharger
Collections
Articles publiés dans des revues avec comité de lecture