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Publication :
RGD and BMP-2 mimetic peptides crosstalk enhances osteogenic commitment of human bone marrow stem cells

bul.description.provenanceemo spbfr
bul.rights.dateAccepPubl2016-03-18fr
bul.rights.periodeEmbargoP2Yfr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorBilem, Ibrahim
dc.contributor.authorLaroche, Gaétan
dc.contributor.authorPlawinski, Laurent
dc.contributor.authorChevallier, Pascale
dc.contributor.authorStone, E.
dc.contributor.authorDurrieu, Marie-Christine
dc.date.accessioned2019-07-23T19:36:32Z
dc.date.available2019-07-23T19:36:32Z
dc.date.issued2016-03-18
dc.description.abstractHuman bone marrow mesenchymal stem cells (hBMSCs) commitment and differentiation are dictated by bioactive molecules sequestered within their Extra Cellular Matrix (ECM). One common approach to mimic the physiological environment is to functionalize biomaterial surfaces with ECM-derived peptides able to recruit stem cells and trigger their linage-specific differentiation. The objective of this work was to investigate combinatorial effects of RGD and BMP-2 mimetic peptides on the osteogenic commitment of hBMSCs, without supplementing the media with pro-osteogenic factors. The RGD peptide promotes cell adhesion via cell transmembrane integrin receptors, while the BMP-2 peptide, corresponding to residues 73-92 of Bone Morphogenetic Protein-2, was shown to induce hBMSCs osteoblast differentiation. The immobilization of peptides on aminated glass was ascertained by X-ray Photoelectron Spectroscopy (XPS), the density of grafted peptides was quantified by fluorescence microscopy and the surface roughness was evaluated using Atomic Force Microscopy (AFM). The osteogenic commitment of hBMSCs cultured on RGD and/or BMP-2 surfaces was characterized by immunohistochemistry using STRO-1 as specific stem cells marker and Runx-2 as an earlier osteogenic marker. Biological results showed that the osteogenic commitment of hBMSCs was enhanced on bifunctionalized surfaces as compared to surfaces containing BMP-2, while on RGD surfaces cells mainly preserved their stemness character. These results demonstrated that RGD and BMP-2 mimetic peptides act synergistically to enhance hBMSCs osteogenesis without supplementing the media with osteogenic factors. These findings contribute to the development of biomimetic materials, allowing a deeper understanding of signaling pathways that govern the transition of stem cells towards the osteoblastic lineage.fr
dc.identifier.doi10.1016/j.actbio.2016.03.032fr
dc.identifier.issn1742-7061fr
dc.identifier.pubmed27000551fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/35599
dc.languageengfr
dc.publisherElsevierfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectStem cellsfr
dc.subjectBiomimetic materialsfr
dc.subjectBone tissue engineeringfr
dc.subjectMimetic peptidesfr
dc.subjectSurface modificationfr
dc.subject.rvmCellules souches mésenchymateusesfr
dc.subject.rvmCellules de moelle osseusefr
dc.subject.rvmGénie tissulairefr
dc.subject.rvmMatériaux biomimétiquesfr
dc.subject.rvmPeptidesfr
dc.titleRGD and BMP-2 mimetic peptides crosstalk enhances osteogenic commitment of human bone marrow stem cellsfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationActa Biomaterialia, Vol. 36, 132–142 (2016)fr
dspace.accessstatus.time2024-03-25 18:01:54
dspace.entity.typePublication
relation.isAuthorOfPublication199149cd-b41d-45fa-b659-28625490c6ec
relation.isAuthorOfPublication7207871e-32e9-4680-a0f1-6ce126aebe57
relation.isAuthorOfPublicationeaba009b-0f2b-4d24-8a89-43d89fce30a9
relation.isAuthorOfPublication.latestForDiscovery199149cd-b41d-45fa-b659-28625490c6ec
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.versionAccepted Manuscript (AM)fr
rioxxterms.version-of-recordhttp://dx.doi.org/10.1016/j.actbio.2016.03.032fr

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