Induction and elimination of prophages using CRISPR interference

Moineau, Sylvain; Cornuault, Jeffrey K.

Publication :
Induction and elimination of prophages using CRISPR interference

bul.description.provenance	noadg spbar	fr
bul.rights.dateAccepPubl	2021-08-16	fr
bul.rights.periodeEmbargo	P1Y	fr
bul.rights.typeDate	datePublication	fr
dc.contributor.author	Moineau, Sylvain
dc.contributor.author	Cornuault, Jeffrey K.
dc.date.accessioned	2021-11-12T15:10:01Z
dc.date.available	2022-08-16
dc.date.issued	2021-08-16
dc.description.abstract	Prophages are widely spread among bacterial genomes, and they can have positive or negative effects on their hosts. A key aspect in the study of prophages is the discovery of their induction signals. Prophage induction can occur by inactivating a phage transcriptional repressor, which is responsible for maintaining the lysogenic state. This repressor can be inactivated through the bacterial SOS response. However, the induction signals for numerous prophages do not involve the SOS system, and therefore significant efforts are needed to identify these conditions. Similarly, curing bacterial strains of inducible prophages is a tedious process, requiring the screening of several colonies. Here, we investigated whether transcriptional silencing of a prophage repressor using CRISPR interference (CRISPRi) would lead to prophage induction. Using Escherichia coli phages λ and P2 as models, we demonstrated the efficiency of CRISPRi for prophage induction and for curing lysogenic strains of their prophages.	fr
dc.identifier.doi	10.1089/crispr.2021.0026	fr
dc.identifier.issn	2573-1599	fr
dc.identifier.pubmed	34406037	fr
dc.identifier.uri	http://hdl.handle.net/20.500.11794/70864
dc.language	eng	fr
dc.publisher	Mary Ann Liebert, Inc	fr
dc.rights	http://purl.org/coar/access_right/c_abf2
dc.subject.rvm	Bactériophages	fr
dc.subject.rvm	Génomes viraux	fr
dc.subject.rvm	Systèmes CRISPR-Cas	fr
dc.title	Induction and elimination of prophages using CRISPR interference	fr
dc.type	article de recherche
dc.type.legacy	COAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherche	fr
dcterms.bibliographicCitation	CRISPR Journal, Vol. 4 (4), 549-557 (2021)	fr
dspace.accessstatus.time	2023-05-25 18:00:28
dspace.entity.type	Publication
relation.isAuthorOfPublication	599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isAuthorOfPublication	bdeadfad-4b37-4c8d-9b3b-d387a86c04d1
relation.isAuthorOfPublication.latestForDiscovery	599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isResourceTypeOfPublication	4c433ef5-3937-4530-8252-cca17d715747
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rioxxterms.project.funder-name	Natural Sciences and Engineering Research Council of Canada	fr
rioxxterms.project.funder-name	Canada First Research Excellence Fund	fr
rioxxterms.version	Accepted Manuscript (AM)	fr
rioxxterms.version-of-record	https://doi.org/10.1089/crispr.2021.0026	fr