Publication :
Induction and elimination of prophages using CRISPR interference

bul.description.provenancenoadg spbarfr
bul.rights.dateAccepPubl2021-08-16fr
bul.rights.periodeEmbargoP1Yfr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorMoineau, Sylvain
dc.contributor.authorCornuault, Jeffrey K.
dc.date.accessioned2021-11-12T15:10:01Z
dc.date.available2022-08-16
dc.date.issued2021-08-16
dc.description.abstractProphages are widely spread among bacterial genomes, and they can have positive or negative effects on their hosts. A key aspect in the study of prophages is the discovery of their induction signals. Prophage induction can occur by inactivating a phage transcriptional repressor, which is responsible for maintaining the lysogenic state. This repressor can be inactivated through the bacterial SOS response. However, the induction signals for numerous prophages do not involve the SOS system, and therefore significant efforts are needed to identify these conditions. Similarly, curing bacterial strains of inducible prophages is a tedious process, requiring the screening of several colonies. Here, we investigated whether transcriptional silencing of a prophage repressor using CRISPR interference (CRISPRi) would lead to prophage induction. Using Escherichia coli phages λ and P2 as models, we demonstrated the efficiency of CRISPRi for prophage induction and for curing lysogenic strains of their prophages.fr
dc.identifier.doi10.1089/crispr.2021.0026fr
dc.identifier.issn2573-1599fr
dc.identifier.pubmed34406037fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/70864
dc.languageengfr
dc.publisherMary Ann Liebert, Incfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.rvmBactériophagesfr
dc.subject.rvmGénomes virauxfr
dc.subject.rvmSystèmes CRISPR-Casfr
dc.titleInduction and elimination of prophages using CRISPR interferencefr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationCRISPR Journal, Vol. 4 (4), 549-557 (2021)fr
dspace.accessstatus.time2023-05-25 18:00:28
dspace.entity.typePublication
relation.isAuthorOfPublication599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isAuthorOfPublicationbdeadfad-4b37-4c8d-9b3b-d387a86c04d1
relation.isAuthorOfPublication.latestForDiscovery599b62d2-4b51-4b07-9539-3cef15723b3f
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.project.funder-nameCanada First Research Excellence Fundfr
rioxxterms.versionAccepted Manuscript (AM)fr
rioxxterms.version-of-recordhttps://doi.org/10.1089/crispr.2021.0026fr
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