Publication :
Characterization of in vitro engineered human adipose tissues : relevant adipokine secretion and impact of TNF-α

bul.description.provenancemcp spbfr
bul.rights.dateAccepPubl2015-09-14fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.audience.peerreview1fr
dc.contributor.authorRoy, Alphonse
dc.contributor.authorProulx, Maryse
dc.contributor.authorCôté, Jean-François
dc.contributor.authorSafoine, Meryem
dc.contributor.authorAubin, Kim
dc.contributor.authorAudet-Casgrain, Marie-Alice
dc.contributor.authorFradette, Julie
dc.contributor.authorTêtu, Félix-André
dc.date.accessioned2018-09-24T19:00:51Z
dc.date.available2018-09-24T19:00:51Z
dc.date.issued2015-09-14
dc.description.abstractRepresentative modelling of human adipose tissue functions is central to metabolic research. Tridimensional models able to recreate human adipogenesis in a physiological tissue-like context in vitro are still scarce. We describe the engineering of white adipose tissues reconstructed from their cultured adipose-derived stromal precursor cells. We hypothesize that these reconstructed tissues can recapitulate key functions of AT under basal and pro-inflammatory conditions. These tissues, featuring human adipocytes surrounded by stroma, were stable and metabolically active in long-term cultures (at least 11 weeks). Secretion of major adipokines and growth factors by the reconstructed tissues was determined and compared to media conditioned by human native fat explants. Interestingly, the secretory profiles of the reconstructed adipose tissues indicated an abundant production of leptin, PAI-1 and angiopoietin-1 proteins, while higher HGF levels were detected for the human fat explants. We next demonstrated the responsiveness of the tissues to the pro-inflammatory stimulus TNF-α, as reflected by modulation of MCP-1, NGF and HGF secretion, while VEGF and leptin protein expression did not vary. TNF-α exposure induced changes in gene expression for adipocyte metabolism-associated mRNAs such as SLC2A4, FASN and LIPE, as well as for genes implicated in NF-κB activation. Finally, this model was customized to feature adipocytes representative of progressive stages of differentiation, thereby allowing investigations using newly differentiated or more mature adipocytes. In conclusion, we produced tridimensional tissues engineered in vitro that are able to recapitulate key characteristics of subcutaneous white adipose tissue. These tissues are produced from human cells and their neo-synthesized matrix elements without exogenous or synthetic biomaterials. Therefore, they represent unique tools to investigate the effects of pharmacologically active products on human stromal cells, extracellular matrix and differentiated adipocytes, in addition to compounds modulating adipogenesis from precursor cells.fr
dc.identifier.doi10.1371/journal.pone.0137612fr
dc.identifier.issn1932-6203fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/31344
dc.languageengfr
dc.publisherPublic Library of Sciencefr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subjectAdipocytesfr
dc.subjectTNF-αfr
dc.subjectAdipokine secretionfr
dc.subjectAdipose tissuefr
dc.subjectSelf-assembly approachfr
dc.subjectTissue engineeringfr
dc.subject.rvmAdipocytesfr
dc.subject.rvmFacteur de nécrose tumorale alphafr
dc.subject.rvmAdipokinesfr
dc.subject.rvmTissu adipeuxfr
dc.subject.rvmGénie tissulairefr
dc.titleCharacterization of in vitro engineered human adipose tissues : relevant adipokine secretion and impact of TNF-αfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationPlosOne, Vol. 10 (9), (2015)fr
dspace.accessstatus.time2023-01-28 18:01:57
dspace.entity.typePublication
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rioxxterms.versionAMfr
rioxxterms.version-of-recordhttp://dx.doi.org/10.1371/journal.pone.0137612fr
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