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Publication :
Dicer-derived microRNAs are utilized by the Fragile X mental retardation protein for assembly on target RNAs

bul.description.provenancemcp spbfr
bul.rights.dateAccepPubl30-05-2006fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedateAcceptationfr
dc.contributor.authorTremblay, Sandra
dc.contributor.authorOuellet, Dominique
dc.contributor.authorKhandjian, Edward William
dc.contributor.authorPlante, Isabelle
dc.contributor.authorProvost, Patrick
dc.contributor.authorDavidovic, Laetitia
dc.contributor.authorGobeil, Lise-Andrée
dc.date.accessioned2019-04-04T19:45:46Z
dc.date.available2019-10-04T00:00:00Z
dc.date.issued2006-09-06
dc.description.abstractIn mammalian cells, fragile X mental retardation protein (FMRP) has been reported to be part of a microRNA (miRNA)-containing effector ribonucleoprotien (RNP) complex believed to mediate translational control of specific mRNAs. Here, using recombinant proteins, we demonstrate that human FMRP can act as a miRNA acceptor protein for the ribonuclease Dicer and facilitate the assembly of miRNAs on specific target RNA sequences. The miRNA assembler property of FMRP was abrogated upon deletion of its single-stranded (ss) RNA binding K-homology domains. The requirement of FMRP for efficient RNA interference (RNAi) in vivo was unveiled by reporter gene silencing assays using various small RNA inducers, which also supports its involvement in an ss small interfering RNA (siRNA)-containing RNP (siRNP) effector complex in mammalian cells. Our results define a possible role for FMRP in RNA silencing and may provide further insight into the molecular defects in patients with the fragile X syndrome.fr
dc.identifier.doi10.1155/JBB/2006/64347fr
dc.identifier.issn1110-7243fr
dc.identifier.pubmed17057366fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/34272
dc.languageengfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.rvmMicroARNfr
dc.subject.rvmSyndrome de l'X fragile -- Aspect génétiquefr
dc.subject.rvmRibonucléase IIIfr
dc.subject.rvmRibonucléoprotéinesfr
dc.subject.rvmInactivation géniquefr
dc.titleDicer-derived microRNAs are utilized by the Fragile X mental retardation protein for assembly on target RNAsfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationJournal of Biomedicine and Biotechnology, Vol. 2006 (2006)fr
dcterms.date.accepted2006-05-30
dspace.accessstatus.time2024-03-23 18:04:55
dspace.entity.typePublication
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rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.project.funder-nameCanadian Institutes of Health Researchfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttp://dx.doi.org/10.1155/JBB/2006/64347fr

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