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Lactococcus lactis type III-A CRISPR-Cas system cleaves bacteriophage RNA

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bul.description.provenancenag spbfr
bul.rights.dateAccepPubl2018-10-02fr
bul.rights.periodeEmbargoforeverfr
bul.rights.raisonEmbargoInfiniPour que le document soit diffusé en libre accès, en accord avec le délai prescrit par l’éditeur de RNA biology, il faudrait déposer la version acceptée pour publication, incluant toutes les modifications demandées, mais sans la mise en page de la revue. Pour ce faire, effectuez une demande de modification à l’aide de la liste des dépôts diffusés à partir du tableau de suivi.fr
bul.rights.typeDatedatePublicationfr
dc.contributor.authorMillen, Anne M.
dc.contributor.authorTremblay, Denise
dc.contributor.authorMoineau, Sylvain
dc.contributor.authorSamson, Julie
dc.contributor.authorMagadán, Alfonso H.
dc.contributor.authorRousseau, Geneviève M.
dc.contributor.authorRomero, Dennis A.
dc.date.accessioned2020-04-21T14:23:36Z
dc.date.available9999-12-31
dc.date.issued2018-10-02
dc.description.abstractCRISPR-Cas defends microbial cells against invading nucleic acids including viral genomes. Recent studies have shown that type III-A CRISPR-Cas systems target both RNA and DNA in a transcriptiondependent manner. We previously found a type III-A system on a conjugative plasmid in Lactococcus lactis which provided resistance against virulent phages of the Siphoviridae family. Its naturally occurring spacers are oriented to generate crRNAs complementary to target phage mRNA, suggesting transcription-dependent targeting. Here, we show that only constructs whose spacers produce crRNAs complementary to the phage mRNA confer phage resistance in L. lactis. In vivo nucleic acid cleavage assays showed that cleavage of phage dsDNA genome was not detected within phage-infected L. lactis cells. On the other hand, Northern blots indicated that the lactococcal CRISPR-Cas cleaves phage mRNA in vivo. These results cannot exclude that single-stranded phage DNA is not being targeted, but phage DNA replication has been shown to be impaired.fr
dc.identifier.doi10.1080/15476286.2018.1502589fr
dc.identifier.issn1547-6286fr
dc.identifier.pubmed30081743fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/38895
dc.languageengfr
dc.publisherTandfonlinefr
dc.rightshttp://purl.org/coar/access_right/c_16ec
dc.subjectPhagesfr
dc.subjectCRISPRfr
dc.subjectLactococcusfr
dc.subjectResistancefr
dc.subject.rvmSystèmes CRISPR-Casfr
dc.subject.rvmLactococcus lactisfr
dc.subject.rvmBactériophagesfr
dc.subject.rvmARN viralfr
dc.titleLactococcus lactis type III-A CRISPR-Cas system cleaves bacteriophage RNAfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationRNA Biology, Vol. 16 (4), 461–468 (2019)fr
dspace.accessstatus.time2023-05-24 18:06:18
dspace.entity.typePublication
relation.isAuthorOfPublication17224655-a23d-48b9-b40d-a5a58328ffde
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relation.isAuthorOfPublication8bafb194-d9a0-423e-a712-ac341354248e
relation.isAuthorOfPublicationb7a60147-0d40-485d-ad96-97ff59b61d9c
relation.isAuthorOfPublicationc9a1a7fa-f824-443f-93f4-f507a736bbfa
relation.isAuthorOfPublication.latestForDiscovery17224655-a23d-48b9-b40d-a5a58328ffde
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rioxxterms.project.funder-nameNatural Sciences and Engineering Research Council of Canadafr
rioxxterms.project.funder-nameautrefr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.1080/15476286.2018.1502589fr
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