Publication :
Autologous transplantation of rabbit limbal epithelia cultured on fibrin gels for ocular surface reconstruction

Notice brève
bul.description.provenanceeb spbfr
bul.rights.dateAccepPubl2006-02-01fr
bul.rights.periodeEmbargoP0Mfr
bul.rights.typeDatedatePublicationfr
dc.audience.peerreview1fr
dc.contributor.authorGuérin, Sylvain
dc.contributor.authorGermain, Lucie
dc.contributor.authorGiasson, Claude J.
dc.contributor.authorGiroux-Talbot, Mariève
dc.contributor.authorAuger, François A.
dc.contributor.authorBazin, Richard
dc.contributor.authorCarrier, Patrick
dc.contributor.authorDeschambeault, Alexandre
dc.date.accessioned2018-01-26T16:41:12Z
dc.date.available2018-01-26T16:41:12Z
dc.date.issued2006-02-01
dc.description.abstractPurpose: Regeneration of the corneal epithelium could be severely impaired in patients suffering from limbal stem cell deficiency. The purpose of this study was to evaluate the restoration of the corneal epithelium by grafting onto denuded corneas autologous limbal cells cultured on fibrin gels. The rabbit model was chosen to allow the microscopic evaluation over time after grafting. Methods: Rabbit limbal epithelial cells (RLECs) were isolated and cultured from small limbal biopsies (3 mm2). The epithelium was separated from stroma after dispase digestion and put in culture on lethally irradiated fibroblasts used as a feeder layer. At the first passage, RLECs were cultured on a fibrin gel matrix. At confluence, the cultured epithelia were grafted in vivo on denuded autologous rabbit corneas. At different postoperative times, grafted and control (without graft or grafted with fibrin gels only) rabbit corneas were compared in vivo with a slit lamp microscope, and in situ by histological and immunohistological microscopy of harvested biopsies. Results: A small limbal biopsy was sufficient to generate enough RLECs to prepare several grafts and to perform cell analysis. Only two weeks were required to produce a cultured epithelium suitable for autologous transplantation. One month after grafting, a normal corneal phenotype was observed on the ocular surface of grafted rabbits in contrast to the control rabbits (ungrafted or grafted with fibrin gel only) where histological signs of conjunctivalization were found. The absence of goblet cells and negative staining for keratin 4 confirmed that the cultured cells persisted and that the epithelium regenerated after grafting was not from conjunctival origin. Conclusions: Our results demonstrate that an autologous epithelium cultured on a physiologically biodegradable matrix can be prepared from a small biopsy and grafted on denuded cornea. The autologous graft allows epithelial regeneration from cultured cells and promotes corneal healing of unilateral total stem cell deficiency.fr
dc.identifier.issn1090-0535fr
dc.identifier.pubmed16479251fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/16689
dc.languageengfr
dc.publisherÉditeur non identifiéfr
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.subject.rvmAutoplastiefr
dc.subject.rvmÉpithélium antérieur de la cornéefr
dc.subject.rvmCellules souchesfr
dc.subject.rvmGreffe (Chirurgie) -- Modèles animauxfr
dc.subject.rvmFibrinefr
dc.subject.rvmLapins (Animaux de laboratoire)fr
dc.titleAutologous transplantation of rabbit limbal epithelia cultured on fibrin gels for ocular surface reconstructionfr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationMolecular vision, Vol. 12 (7), 65–75 (2006)fr
dspace.accessstatus.time2023-05-24 18:05:59
dspace.entity.typePublication
relation.isAuthorOfPublication2ade4e31-1fd3-415e-8e7d-fdda42023eb6
relation.isAuthorOfPublication375ce2c4-1171-4ecc-9901-7b68e028f911
relation.isAuthorOfPublication8e166d76-d471-41c6-80bf-6e45c51740f3
relation.isAuthorOfPublication978ec9c6-43e3-4ecb-8f9c-81c1c6b1062a
relation.isAuthorOfPublication9a76c882-fb54-4a05-ad1c-b75fe72827ed
relation.isAuthorOfPublicationd653af21-8c59-438d-bdb0-4b3d7270a0cc
relation.isAuthorOfPublicationfb3047b0-81d9-47e5-9067-35fe783b7714
relation.isAuthorOfPublication.latestForDiscovery2ade4e31-1fd3-415e-8e7d-fdda42023eb6
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
relation.isResourceTypeOfPublication.latestForDiscovery4c433ef5-3937-4530-8252-cca17d715747
rioxxterms.project.funder-nameCanadian Institutes of Health Researchfr
rioxxterms.project.funder-nameFonds de Recherche du Québec - Santéfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttp://www.molvis.org/molvis/v12/a7/fr
Fichiers
Bundle original
Voici les éléments 1 - 1 sur 1
Vignette d'image
Nom :
2006_Talbot_MolecVision.pdf
Taille :
348.34 KB
Format :
Adobe Portable Document Format
Description :
Télécharger
Collections
Articles publiés dans des revues avec comité de lecture