Publication :
A novel approach for studying angiogenesis : a human skin equivalent with a capillary-like network

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Date
1999-04-01
Direction de publication
Direction de recherche
Titre de la revue
ISSN de la revue
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Éditeur
Princeton Scientific Publishers
Projets de recherche
Structures organisationnelles
Numéro de revue
Résumé
Angiogenesis results from an ordered set of events that can be modulated in vivo by a variety of angiogenesis-enhancing or inhibiting agents. We review in vitro angiogenesis models and the agents that enhance or inhibit angiogenesis. We also discuss a new in vitro angiogenesis model created within a skin equivalent. Briefly, endothelial cells were combined with the cutaneous cells of a standard skin equivalent and cultured in a chitosan cross-linked collagen-glycosaminoglycan scaffold of this endothelialized skin. This model enables the formation of capillary-like structures in a coculture environment containing newly synthesized extracellular matrix by fibroblasts and keratinocytes. Several morphological characteristics associated with the microvasculature in vivo were observed in the endothelialized skin equivalent such as histotypic organization of tubular structures, basement membrane deposition, and intercellular junction formation.
Description
Revue
Cell Biology and Toxicology, Vol. 15 (2), 81-90 (1999)
DOI
10.1023/A:1007541713398
URL vers la version publiée
Mots-clés
Angiogenesis , Endothelial cell , Extracellular matrix , Skin equivalent
Citation
Type de document
article de recherche