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Publication :
Vibrissa hair bulge houses two populations of skin epithelial stem cells distinct by their keratin profile

bul.description.provenancelf spbfr
bul.rights.dateAccepPubl2007-12-27fr
bul.rights.periodeEmbargoforeverfr
bul.rights.raisonEmbargoInfiniL’éditeur de FASEB journal n’autorise pas la diffusion en libre accès dans un dépôt institutionnel.fr
bul.rights.typeDatedatePublicationfr
dc.audience.peerreview1fr
dc.contributor.authorGermain, Lucie
dc.contributor.authorTong, Xuemei
dc.contributor.authorLarouche, Danielle
dc.contributor.authorFradette, Julie
dc.contributor.authorCoulombe, Pierre A.
dc.date.accessioned2018-01-29T15:05:22Z
dc.date.available9999-12-31
dc.date.issued2007-12-27
dc.description.abstractDefining the properties of postnatal stem cells is of interest given their relevance for tissue homeostasis and therapeutic applications, such as skin tissue engineering for burn patients. In hair follicles, the bulge region of the outer root sheath houses stem cells. We show that explants from the prominent bulge area, but not the bulb, in rodent vibrissa follicles can produce epidermis in a skin model of tissue engineering. Using morphological criteria and keratin expression, we typified epithelial stem cells of vibrissa bulge. Two types of slow-cycling cells (Bb, Bs1) featuring a high colony-forming capacity occur in the bulge. Bb cells are located in the outermost basal layer, express K5, K15, K17, and K19, and feature a loosely organized keratin network. Bs1 cells localize to the suprabasal layers proximal to Bb cells and express K5/K17, corre lating with a network of densely bundled filaments. These prominent bundles are missing in K17-null mice, which lack vibrissa. Atypically, both the Bb and Bs1 keratinocytes lack K14 expression. These findings show heterogeneity within the hair follicle stem cell reposi tory, establish that a subset of slow-cycling cells are suprabasal in location, and point to a special role for K5/K17 filaments in a newly defined subset of stem cells. Our results are discussed in the context of long-term survival of engineered tissues after grafting that requires the presence of stem cells.fr
dc.identifier.doi10.1096/fj.07-8109comfr
dc.identifier.issn0892-6638fr
dc.identifier.pubmed18162489fr
dc.identifier.urihttp://hdl.handle.net/20.500.11794/16726
dc.languageengfr
dc.publisherFederation of American Societies for Experimental Biologyfr
dc.rightshttp://purl.org/coar/access_right/c_16ec
dc.subjectIntermediate filamentsfr
dc.subjectTissue engineeringfr
dc.subjectTransmission electron microscopyfr
dc.subjectRodentfr
dc.subject.rvmFilaments intermédiairesfr
dc.subject.rvmGénie tissulairefr
dc.subject.rvmMicroscopie électronique à transmissionfr
dc.subject.rvmCellules souchesfr
dc.subject.rvmFollicule pileuxfr
dc.subject.rvmSouris (Animal de laboratoire)fr
dc.subject.rvmVibrissesfr
dc.titleVibrissa hair bulge houses two populations of skin epithelial stem cells distinct by their keratin profilefr
dc.typearticle de recherche
dc.type.legacyCOAR1_1::Texte::Périodique::Revue::Contribution à un journal::Article::Article de recherchefr
dcterms.bibliographicCitationThe FASEB journal, Vol. 22 (5), 1404-1415 (2008)fr
dspace.accessstatus.time2024-03-23 18:03:29
dspace.entity.typePublication
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relation.isAuthorOfPublication4559dc8d-fb9e-4e20-8117-d4999e7a1c52
relation.isAuthorOfPublicationf01dce1c-fb36-4d77-8977-5c6058eff4a3
relation.isAuthorOfPublication.latestForDiscovery375ce2c4-1171-4ecc-9901-7b68e028f911
relation.isResourceTypeOfPublication4c433ef5-3937-4530-8252-cca17d715747
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rioxxterms.project.funder-nameCanadian Institutes of Health Researchfr
rioxxterms.project.funder-nameNational Institute of Arthritis and Musculoskeletal and Skin Diseasesfr
rioxxterms.versionVersion of Record (VoR)fr
rioxxterms.version-of-recordhttps://doi.org/10.1096/fj.07-8109comfr

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