Publication :
Cyclic traction machine for long-term culture of fibroblast-populated collagen gels

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Date
1999-01-01
Direction de publication
Direction de recherche
Titre de la revue
ISSN de la revue
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Éditeur
Springer
Projets de recherche
Structures organisationnelles
Numéro de revue
Résumé
Our research group has been investigating the effect of cyclic deformations on the evolution of fibroblast populated collagen gels (FPCG). Since existing traction machines are not designed for such an application, we had to design a cyclic traction machine adapted to tissue culture inside an incubator over an extended period of time. Biocompatible materials were used for fabrication to allow for easy sterilization and to prevent any adverse reaction from the tissue. The traction machine is based on a computer-controlled stepping motor system for easy adjustment of the deformation amplitude and frequency. The maximum stretching speed achieved is around 1mm/s. The traction machine can measure FPCG mechanical properties and perform rupture tests to determine its ultimate strength. Several FPCGs have been successfully cultured with the machine for up to four weeks without any adverse reaction.
Description
Revue
Annals of Biomedical Engineering, Vol. 27 (1), 67-72 (1999)
DOI
10.1114/1.166
URL vers la version publiée
Mots-clés
Tissue engineering , Collagen gels , Fibroblasts , Cyclic traction , Traction machine , Collagen fibers orientation
Citation
Type de document
article de recherche